Neuropilin-1 is a host factor for SARS-CoV-2 infection

James Daly, Boris Simonetti*, Katja Klein, Maia Kavanagh Williamson, Carlos Anton Plagaro, Deborah K Shoemark, Richard B Sessions, David A Matthews, Andrew D Davidson, Pete J Cullen *, Yohei Yamauchi*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

833 Citations (Scopus)
200 Downloads (Pure)


SARS-CoV-2, the causative agent of COVID-19, uses the viral Spike (S) protein for host cell attachment and entry. The host protease furin cleaves the full-length precursor S glycoprotein into two associated polypeptides: S1 and S2. Cleavage of S generates a polybasic Arg-Arg-Ala-Arg C-terminal sequence on S1, which conforms to a C-end rule (CendR) motif that binds to cell surface Neuropilin-1 (NRP1) and Neuropilin-2 (NRP2) receptors. Here, we used X-ray crystallography and biochemical approaches to show that the S1 CendR motif directly bound NRP1. Blocking this interaction using RNAi or selective inhibitors reduced SARS-CoV-2 entry and infectivity in cell culture. NRP1 thus serves as a host factor for SARS-CoV-2 infection and may potentially provide a therapeutic target for COVID-19.
Original languageEnglish
Article numbereabd3072
Pages (from-to)861-865
Number of pages5
Issue number6518
Early online date20 Oct 2020
Publication statusPublished - 13 Nov 2020

Structured keywords

  • BrisSynBio
  • Covid19
  • Bristol BioDesign Institute


Dive into the research topics of 'Neuropilin-1 is a host factor for SARS-CoV-2 infection'. Together they form a unique fingerprint.

Cite this