Neuroprotection in a rat Parkinson model by GDNF gene therapy using EIAV vector

M Azzouz; S Ralph; LF Wong; D Day; Z Askham; RD Barber; [No Value] Mitrophanous KA; SM Kingsman; ND Mazarakis

Neuroprotection in a rat Parkinson model by GDNF gene therapy using EIAV vector

M Azzouz, S Ralph, LF Wong, D Day, Z Askham, RD Barber, [No Value] Mitrophanous KA, SM Kingsman, ND Mazarakis

Research output: Contribution to journal › Article (Academic Journal) › peer-review

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Abstract

Vectors based on lentiviruses are opening up new approaches for the treatment of neurodegenerative diseases. Currently, the equine infectious anaemia virus (EIAV) vector is one of the most attractive gene delivery systems with respect to neuronal tropism. The aim was to validate EIAV-lentiviral vectors as a gene delivery system for neurotrophic factor genes in an animal model of Parkinson's disease. EIAV carrying the glial cell line-derived neurotrophic factor (GDNF) gene was unilaterally injected into rat striatum and above the substantia nigra (SN). One week later, the rats received a 6-OHDA lesion into the ipsilateral striatum. GDNF delivery led to extensive expression of GDNF protein within the striatum. In addition, near complete protection against dopaminergic cell death was observed in the GDNF-treated group.

Translated title of the contribution	Neuroprotection in a rat Parkinson model by GDNF gene therapy using EIAV vector
Original language	English
Pages (from-to)	985 - 990
Journal	NeuroReport
Volume	15(6)
Publication status	Published - 2004

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@article{a51c3b36237d4988bf2024f335e1495b,

title = "Neuroprotection in a rat Parkinson model by GDNF gene therapy using EIAV vector",

abstract = "Vectors based on lentiviruses are opening up new approaches for the treatment of neurodegenerative diseases. Currently, the equine infectious anaemia virus (EIAV) vector is one of the most attractive gene delivery systems with respect to neuronal tropism. The aim was to validate EIAV-lentiviral vectors as a gene delivery system for neurotrophic factor genes in an animal model of Parkinson's disease. EIAV carrying the glial cell line-derived neurotrophic factor (GDNF) gene was unilaterally injected into rat striatum and above the substantia nigra (SN). One week later, the rats received a 6-OHDA lesion into the ipsilateral striatum. GDNF delivery led to extensive expression of GDNF protein within the striatum. In addition, near complete protection against dopaminergic cell death was observed in the GDNF-treated group.",

author = "M Azzouz and S Ralph and LF Wong and D Day and Z Askham and RD Barber and {Mitrophanous KA}, {[No Value]} and SM Kingsman and ND Mazarakis",

year = "2004",

language = "English",

volume = "15(6)",

pages = "985 -- 990",

journal = "NeuroReport",

issn = "1473-558X",

publisher = "Lippincott Williams and Wilkins",

}

TY - JOUR

T1 - Neuroprotection in a rat Parkinson model by GDNF gene therapy using EIAV vector

AU - Azzouz, M

AU - Ralph, S

AU - Wong, LF

AU - Day, D

AU - Askham, Z

AU - Barber, RD

AU - Mitrophanous KA, [No Value]

AU - Kingsman, SM

AU - Mazarakis, ND

PY - 2004

Y1 - 2004

N2 - Vectors based on lentiviruses are opening up new approaches for the treatment of neurodegenerative diseases. Currently, the equine infectious anaemia virus (EIAV) vector is one of the most attractive gene delivery systems with respect to neuronal tropism. The aim was to validate EIAV-lentiviral vectors as a gene delivery system for neurotrophic factor genes in an animal model of Parkinson's disease. EIAV carrying the glial cell line-derived neurotrophic factor (GDNF) gene was unilaterally injected into rat striatum and above the substantia nigra (SN). One week later, the rats received a 6-OHDA lesion into the ipsilateral striatum. GDNF delivery led to extensive expression of GDNF protein within the striatum. In addition, near complete protection against dopaminergic cell death was observed in the GDNF-treated group.

AB - Vectors based on lentiviruses are opening up new approaches for the treatment of neurodegenerative diseases. Currently, the equine infectious anaemia virus (EIAV) vector is one of the most attractive gene delivery systems with respect to neuronal tropism. The aim was to validate EIAV-lentiviral vectors as a gene delivery system for neurotrophic factor genes in an animal model of Parkinson's disease. EIAV carrying the glial cell line-derived neurotrophic factor (GDNF) gene was unilaterally injected into rat striatum and above the substantia nigra (SN). One week later, the rats received a 6-OHDA lesion into the ipsilateral striatum. GDNF delivery led to extensive expression of GDNF protein within the striatum. In addition, near complete protection against dopaminergic cell death was observed in the GDNF-treated group.

M3 - Article (Academic Journal)

SN - 1473-558X

VL - 15(6)

SP - 985

EP - 990

JO - NeuroReport

JF - NeuroReport

ER -

Neuroprotection in a rat Parkinson model by GDNF gene therapy using EIAV vector

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