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Neutral lineage tracing of proliferative embryonic and adult mammary stem/progenitor cells

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Neutral lineage tracing of proliferative embryonic and adult mammary stem/progenitor cells. / Lloyd-Lewis, Bethan; Davis, Felicity M; Harris, Olivia B; Hitchcock, Jessica R; Watson, Christine J.

In: Development (Cambridge), Vol. 145, dev164079, 25.07.2018.

Research output: Contribution to journalArticle

Harvard

Lloyd-Lewis, B, Davis, FM, Harris, OB, Hitchcock, JR & Watson, CJ 2018, 'Neutral lineage tracing of proliferative embryonic and adult mammary stem/progenitor cells', Development (Cambridge), vol. 145, dev164079. https://doi.org/10.1242/dev.164079

APA

Lloyd-Lewis, B., Davis, F. M., Harris, O. B., Hitchcock, J. R., & Watson, C. J. (2018). Neutral lineage tracing of proliferative embryonic and adult mammary stem/progenitor cells. Development (Cambridge), 145, [dev164079]. https://doi.org/10.1242/dev.164079

Vancouver

Lloyd-Lewis B, Davis FM, Harris OB, Hitchcock JR, Watson CJ. Neutral lineage tracing of proliferative embryonic and adult mammary stem/progenitor cells. Development (Cambridge). 2018 Jul 25;145. dev164079. https://doi.org/10.1242/dev.164079

Author

Lloyd-Lewis, Bethan ; Davis, Felicity M ; Harris, Olivia B ; Hitchcock, Jessica R ; Watson, Christine J. / Neutral lineage tracing of proliferative embryonic and adult mammary stem/progenitor cells. In: Development (Cambridge). 2018 ; Vol. 145.

Bibtex

@article{b6edb115d3184e6da94164e13177f2de,
title = "Neutral lineage tracing of proliferative embryonic and adult mammary stem/progenitor cells",
abstract = "Mammary gland development occurs over multiple phases, beginning in the mammalian embryo and continuing throughout reproductive life. The remarkable morphogenetic capacity of the mammary gland at each stage of development is attributed to the activities of distinct populations of mammary stem cells (MaSCs) and progenitor cells. However, the relationship between embryonic and adult MaSCs, and their fate during different waves of mammary gland morphogenesis, remains unclear. By employing a neutral, low-density genetic labelling strategy, we characterised the contribution of proliferative stem/progenitor cells to embryonic, pubertal and reproductive mammary gland development. Our findings further support a model of lineage restriction of MaSCs in the postnatal mammary gland, and highlight extensive redundancy and heterogeneity within the adult stem/progenitor cell pool. Furthermore, our data suggest extensive multiplicity in their foetal precursors that give rise to the primordial mammary epithelium before birth. In addition, using a single-cell labelling approach, we revealed the extraordinary capacity of a single embryonic MaSC to contribute to postnatal ductal development. Together, these findings provide tantalising new insights into the disparate and stage-specific contribution of distinct stem/progenitor cells to mammary gland development.",
keywords = "Adult Stem Cells/cytology, Animals, Cell Lineage, Cell Proliferation, Embryonic Development, Mammary Glands, Animal/cytology, Mice, Morphogenesis, Mouse Embryonic Stem Cells/cytology, Sexual Maturation, Single-Cell Analysis",
author = "Bethan Lloyd-Lewis and Davis, {Felicity M} and Harris, {Olivia B} and Hitchcock, {Jessica R} and Watson, {Christine J}",
note = "{\circledC} 2018. Published by The Company of Biologists Ltd.",
year = "2018",
month = "7",
day = "25",
doi = "10.1242/dev.164079",
language = "English",
volume = "145",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - Neutral lineage tracing of proliferative embryonic and adult mammary stem/progenitor cells

AU - Lloyd-Lewis, Bethan

AU - Davis, Felicity M

AU - Harris, Olivia B

AU - Hitchcock, Jessica R

AU - Watson, Christine J

N1 - © 2018. Published by The Company of Biologists Ltd.

PY - 2018/7/25

Y1 - 2018/7/25

N2 - Mammary gland development occurs over multiple phases, beginning in the mammalian embryo and continuing throughout reproductive life. The remarkable morphogenetic capacity of the mammary gland at each stage of development is attributed to the activities of distinct populations of mammary stem cells (MaSCs) and progenitor cells. However, the relationship between embryonic and adult MaSCs, and their fate during different waves of mammary gland morphogenesis, remains unclear. By employing a neutral, low-density genetic labelling strategy, we characterised the contribution of proliferative stem/progenitor cells to embryonic, pubertal and reproductive mammary gland development. Our findings further support a model of lineage restriction of MaSCs in the postnatal mammary gland, and highlight extensive redundancy and heterogeneity within the adult stem/progenitor cell pool. Furthermore, our data suggest extensive multiplicity in their foetal precursors that give rise to the primordial mammary epithelium before birth. In addition, using a single-cell labelling approach, we revealed the extraordinary capacity of a single embryonic MaSC to contribute to postnatal ductal development. Together, these findings provide tantalising new insights into the disparate and stage-specific contribution of distinct stem/progenitor cells to mammary gland development.

AB - Mammary gland development occurs over multiple phases, beginning in the mammalian embryo and continuing throughout reproductive life. The remarkable morphogenetic capacity of the mammary gland at each stage of development is attributed to the activities of distinct populations of mammary stem cells (MaSCs) and progenitor cells. However, the relationship between embryonic and adult MaSCs, and their fate during different waves of mammary gland morphogenesis, remains unclear. By employing a neutral, low-density genetic labelling strategy, we characterised the contribution of proliferative stem/progenitor cells to embryonic, pubertal and reproductive mammary gland development. Our findings further support a model of lineage restriction of MaSCs in the postnatal mammary gland, and highlight extensive redundancy and heterogeneity within the adult stem/progenitor cell pool. Furthermore, our data suggest extensive multiplicity in their foetal precursors that give rise to the primordial mammary epithelium before birth. In addition, using a single-cell labelling approach, we revealed the extraordinary capacity of a single embryonic MaSC to contribute to postnatal ductal development. Together, these findings provide tantalising new insights into the disparate and stage-specific contribution of distinct stem/progenitor cells to mammary gland development.

KW - Adult Stem Cells/cytology

KW - Animals

KW - Cell Lineage

KW - Cell Proliferation

KW - Embryonic Development

KW - Mammary Glands, Animal/cytology

KW - Mice

KW - Morphogenesis

KW - Mouse Embryonic Stem Cells/cytology

KW - Sexual Maturation

KW - Single-Cell Analysis

U2 - 10.1242/dev.164079

DO - 10.1242/dev.164079

M3 - Article

C2 - 30045917

VL - 145

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

M1 - dev164079

ER -