Neutrophil Extracellular Trap Formation Is Independent of De Novo Gene Expression

Gabriel Sollberger, Borko Amulic, Arturo Zychlinsky

Research output: Contribution to journalArticle (Academic Journal)peer-review

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Abstract

Neutrophils are essential innate immune cells whose responses are crucial in the clearance of invading pathogens. Neutrophils can respond to infection by releasing neutrophil extracellular traps (NETs). NETs are formed of chromatin and specific granular proteins and are released after execution of a poorly characterized cell death pathway. Here, we show that NET formation induced by PMA or Candida albicans is independent of RNA polymerase II and III-mediated transcription as well as of protein synthesis. Thus, neutrophils contain all the factors required for NET formation when they emerge from the bone marrow as differentiated cells.

Original languageEnglish
Pages (from-to)e0157454
JournalPLoS ONE
Volume11
Issue number6
Early online date16 Jun 2016
DOIs
Publication statusPublished - 16 Jun 2016

Keywords

  • Animals
  • Candida albicans
  • Chemokine CCL3
  • Cycloheximide
  • Dactinomycin
  • Extracellular Traps
  • Flavonoids
  • Gene Expression Regulation
  • Humans
  • Interleukin-8
  • Lipopolysaccharides
  • Male
  • Mice
  • Neutrophils
  • Piperidines
  • Primary Cell Culture
  • RNA Polymerase II
  • RNA Polymerase III
  • Salmonella typhimurium
  • Tetradecanoylphorbol Acetate
  • Journal Article

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