Neutrophil Extracellular Trap Formation Is Independent of De Novo Gene Expression

Gabriel Sollberger; Borko Amulic; Arturo Zychlinsky

doi:10.1371/journal.pone.0157454

Neutrophil Extracellular Trap Formation Is Independent of De Novo Gene Expression

Gabriel Sollberger, Borko Amulic, Arturo Zychlinsky

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Abstract

Neutrophils are essential innate immune cells whose responses are crucial in the clearance of invading pathogens. Neutrophils can respond to infection by releasing neutrophil extracellular traps (NETs). NETs are formed of chromatin and specific granular proteins and are released after execution of a poorly characterized cell death pathway. Here, we show that NET formation induced by PMA or Candida albicans is independent of RNA polymerase II and III-mediated transcription as well as of protein synthesis. Thus, neutrophils contain all the factors required for NET formation when they emerge from the bone marrow as differentiated cells.

Original language	English
Pages (from-to)	e0157454
Journal	PLoS ONE
Volume	11
Issue number	6
Early online date	16 Jun 2016
DOIs	https://doi.org/10.1371/journal.pone.0157454
Publication status	Published - 16 Jun 2016

Keywords

Animals
Candida albicans
Chemokine CCL3
Cycloheximide
Dactinomycin
Extracellular Traps
Flavonoids
Gene Expression Regulation
Humans
Interleukin-8
Lipopolysaccharides
Male
Mice
Neutrophils
Piperidines
Primary Cell Culture
RNA Polymerase II
RNA Polymerase III
Salmonella typhimurium
Tetradecanoylphorbol Acetate
Journal Article

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Dr Borko Amulic
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- School of Cellular and Molecular Medicine - Associate Professor in Immunology
- Infection and Immunity
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title = "Neutrophil Extracellular Trap Formation Is Independent of De Novo Gene Expression",

abstract = "Neutrophils are essential innate immune cells whose responses are crucial in the clearance of invading pathogens. Neutrophils can respond to infection by releasing neutrophil extracellular traps (NETs). NETs are formed of chromatin and specific granular proteins and are released after execution of a poorly characterized cell death pathway. Here, we show that NET formation induced by PMA or Candida albicans is independent of RNA polymerase II and III-mediated transcription as well as of protein synthesis. Thus, neutrophils contain all the factors required for NET formation when they emerge from the bone marrow as differentiated cells.",

keywords = "Animals, Candida albicans, Chemokine CCL3, Cycloheximide, Dactinomycin, Extracellular Traps, Flavonoids, Gene Expression Regulation, Humans, Interleukin-8, Lipopolysaccharides, Male, Mice, Neutrophils, Piperidines, Primary Cell Culture, RNA Polymerase II, RNA Polymerase III, Salmonella typhimurium, Tetradecanoylphorbol Acetate, Journal Article",

author = "Gabriel Sollberger and Borko Amulic and Arturo Zychlinsky",

year = "2016",

month = jun,

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doi = "10.1371/journal.pone.0157454",

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volume = "11",

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TY - JOUR

T1 - Neutrophil Extracellular Trap Formation Is Independent of De Novo Gene Expression

AU - Sollberger, Gabriel

AU - Amulic, Borko

AU - Zychlinsky, Arturo

PY - 2016/6/16

Y1 - 2016/6/16

N2 - Neutrophils are essential innate immune cells whose responses are crucial in the clearance of invading pathogens. Neutrophils can respond to infection by releasing neutrophil extracellular traps (NETs). NETs are formed of chromatin and specific granular proteins and are released after execution of a poorly characterized cell death pathway. Here, we show that NET formation induced by PMA or Candida albicans is independent of RNA polymerase II and III-mediated transcription as well as of protein synthesis. Thus, neutrophils contain all the factors required for NET formation when they emerge from the bone marrow as differentiated cells.

AB - Neutrophils are essential innate immune cells whose responses are crucial in the clearance of invading pathogens. Neutrophils can respond to infection by releasing neutrophil extracellular traps (NETs). NETs are formed of chromatin and specific granular proteins and are released after execution of a poorly characterized cell death pathway. Here, we show that NET formation induced by PMA or Candida albicans is independent of RNA polymerase II and III-mediated transcription as well as of protein synthesis. Thus, neutrophils contain all the factors required for NET formation when they emerge from the bone marrow as differentiated cells.

KW - Animals

KW - Candida albicans

KW - Chemokine CCL3

KW - Cycloheximide

KW - Dactinomycin

KW - Extracellular Traps

KW - Flavonoids

KW - Gene Expression Regulation

KW - Humans

KW - Interleukin-8

KW - Lipopolysaccharides

KW - Male

KW - Mice

KW - Neutrophils

KW - Piperidines

KW - Primary Cell Culture

KW - RNA Polymerase II

KW - RNA Polymerase III

KW - Salmonella typhimurium

KW - Tetradecanoylphorbol Acetate

KW - Journal Article

U2 - 10.1371/journal.pone.0157454

DO - 10.1371/journal.pone.0157454

M3 - Article (Academic Journal)

C2 - 27310721

SN - 1932-6203

VL - 11

SP - e0157454

JO - PLoS ONE

JF - PLoS ONE

IS - 6

ER -

Neutrophil Extracellular Trap Formation Is Independent of De Novo Gene Expression

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Keywords

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