Noise induces up-regulation of P2X2 receptor subunit of ATP-gated ion channels in the rat cochlea

Julie C-C Wang; Nicholas P Raybould; Lin Luo; Allen F Ryan; Mark B Cannell; Peter R Thorne; Gary D Housley

Noise induces up-regulation of P2X2 receptor subunit of ATP-gated ion channels in the rat cochlea

Julie C-C Wang, Nicholas P Raybould, Lin Luo, Allen F Ryan, Mark B Cannell, Peter R Thorne, Gary D Housley

School of Physiology, Pharmacology & Neuroscience

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Abstract

Regulation of P2X(2) receptor (P2X(2)R) expression in the rat cochlea in response to noise was analysed. Sustained loud sound (90-120 dB white noise, > 6 h), increased P2X(2)R mRNA and protein levels in rat organ of Corti and spiral ganglion (primary auditory neurones). P2X(2)R expression by the type I spiral ganglion neurones, which innervate the inner hair cells via the inner spiral plexus, was confirmed by confocal immunofluorescence. This also revealed increased P2X(2)R labelling of outer hair cell (OHC) stereocilia and cuticular plates, reflecting trafficking of greater numbers of ATP-gated ion channels assembled with P2X(2)R subunits to the transducer site. Whole-cell voltage clamp of OHC confirmed the noise-induced up-regulation of ATP-gated inward currents. These data indicate that regulation of P2X(2) receptor gene expression in the cochlea is adaptive, with sustained loud sound promoting increased transcription and translation specifically at sites regulating hearing sensitivity and auditory neurotrans-mission.

Original language	English
Pages (from-to)	817-823
Number of pages	7
Journal	NeuroReport
Volume	14
Issue number	6
Publication status	Published - 6 Jan 2003

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@article{6a373efc9c9e44dbb143ba1824e27a4e,

title = "Noise induces up-regulation of P2X2 receptor subunit of ATP-gated ion channels in the rat cochlea",

abstract = "Regulation of P2X(2) receptor (P2X(2)R) expression in the rat cochlea in response to noise was analysed. Sustained loud sound (90-120 dB white noise, > 6 h), increased P2X(2)R mRNA and protein levels in rat organ of Corti and spiral ganglion (primary auditory neurones). P2X(2)R expression by the type I spiral ganglion neurones, which innervate the inner hair cells via the inner spiral plexus, was confirmed by confocal immunofluorescence. This also revealed increased P2X(2)R labelling of outer hair cell (OHC) stereocilia and cuticular plates, reflecting trafficking of greater numbers of ATP-gated ion channels assembled with P2X(2)R subunits to the transducer site. Whole-cell voltage clamp of OHC confirmed the noise-induced up-regulation of ATP-gated inward currents. These data indicate that regulation of P2X(2) receptor gene expression in the cochlea is adaptive, with sustained loud sound promoting increased transcription and translation specifically at sites regulating hearing sensitivity and auditory neurotrans-mission.",

author = "Wang, {Julie C-C} and Raybould, {Nicholas P} and Lin Luo and Ryan, {Allen F} and Cannell, {Mark B} and Thorne, {Peter R} and Housley, {Gary D}",

year = "2003",

month = jan,

day = "6",

language = "English",

volume = "14",

pages = "817--823",

journal = "NeuroReport",

issn = "0959-4965",

publisher = "Lippincott Williams and Wilkins",

number = "6",

}

TY - JOUR

T1 - Noise induces up-regulation of P2X2 receptor subunit of ATP-gated ion channels in the rat cochlea

AU - Wang, Julie C-C

AU - Raybould, Nicholas P

AU - Luo, Lin

AU - Ryan, Allen F

AU - Cannell, Mark B

AU - Thorne, Peter R

AU - Housley, Gary D

PY - 2003/1/6

Y1 - 2003/1/6

N2 - Regulation of P2X(2) receptor (P2X(2)R) expression in the rat cochlea in response to noise was analysed. Sustained loud sound (90-120 dB white noise, > 6 h), increased P2X(2)R mRNA and protein levels in rat organ of Corti and spiral ganglion (primary auditory neurones). P2X(2)R expression by the type I spiral ganglion neurones, which innervate the inner hair cells via the inner spiral plexus, was confirmed by confocal immunofluorescence. This also revealed increased P2X(2)R labelling of outer hair cell (OHC) stereocilia and cuticular plates, reflecting trafficking of greater numbers of ATP-gated ion channels assembled with P2X(2)R subunits to the transducer site. Whole-cell voltage clamp of OHC confirmed the noise-induced up-regulation of ATP-gated inward currents. These data indicate that regulation of P2X(2) receptor gene expression in the cochlea is adaptive, with sustained loud sound promoting increased transcription and translation specifically at sites regulating hearing sensitivity and auditory neurotrans-mission.

AB - Regulation of P2X(2) receptor (P2X(2)R) expression in the rat cochlea in response to noise was analysed. Sustained loud sound (90-120 dB white noise, > 6 h), increased P2X(2)R mRNA and protein levels in rat organ of Corti and spiral ganglion (primary auditory neurones). P2X(2)R expression by the type I spiral ganglion neurones, which innervate the inner hair cells via the inner spiral plexus, was confirmed by confocal immunofluorescence. This also revealed increased P2X(2)R labelling of outer hair cell (OHC) stereocilia and cuticular plates, reflecting trafficking of greater numbers of ATP-gated ion channels assembled with P2X(2)R subunits to the transducer site. Whole-cell voltage clamp of OHC confirmed the noise-induced up-regulation of ATP-gated inward currents. These data indicate that regulation of P2X(2) receptor gene expression in the cochlea is adaptive, with sustained loud sound promoting increased transcription and translation specifically at sites regulating hearing sensitivity and auditory neurotrans-mission.

M3 - Article (Academic Journal)

VL - 14

SP - 817

EP - 823

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 6

ER -

Noise induces up-regulation of P2X2 receptor subunit of ATP-gated ion channels in the rat cochlea

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