TY - JOUR
T1 - Non-covalent Immobilization of Desmoplakin Plakin Domain Molecules by Size-Selected Clusters for AFM Imaging
AU - Rodríguez-Zamora, P.
AU - Barreto, J.
AU - Yin, F.
AU - Palmer, R. E.
PY - 2014
Y1 - 2014
N2 - We exploit the immobilization of proteins by size-selected atomic gold clusters to examine the plakin domain of desmoplakin (PD), a human desmosomal protein that is mutated in severe skin diseases and cardiomyopathy, using atomic force microscopy (AFM) in the liquid phase. Contact mode AFM indicates that the PD does not establish a covalent bond size-selected gold nanocluster supported on a graphite substrate, but tapping mode AFM images indicate enhanced weak adsorption. The protein dimensions obtained are compared with predicted values based on electron microscopy, small-angle X-ray scattering (SAXS) and X-ray crystallographic studies.
AB - We exploit the immobilization of proteins by size-selected atomic gold clusters to examine the plakin domain of desmoplakin (PD), a human desmosomal protein that is mutated in severe skin diseases and cardiomyopathy, using atomic force microscopy (AFM) in the liquid phase. Contact mode AFM indicates that the PD does not establish a covalent bond size-selected gold nanocluster supported on a graphite substrate, but tapping mode AFM images indicate enhanced weak adsorption. The protein dimensions obtained are compared with predicted values based on electron microscopy, small-angle X-ray scattering (SAXS) and X-ray crystallographic studies.
KW - Atomic force microscopy
KW - Desmoplakin plakin domain
KW - Protein immobilization
KW - Size-selected clusters
UR - http://www.scopus.com/inward/record.url?scp=84899668028&partnerID=8YFLogxK
U2 - 10.1007/s12668-014-0126-9
DO - 10.1007/s12668-014-0126-9
M3 - Article (Academic Journal)
AN - SCOPUS:84899668028
VL - 4
SP - 97
EP - 103
JO - BioNanoScience
JF - BioNanoScience
SN - 2191-1630
IS - 2
ER -