Nongenetic Reprogramming of a Fungal Highly Reducing Polyketide Synthase

Ahmed A. Yakasai, Jack Davison, Zahida Wasil, Laura M. Halo, Craig P. Butts, Colin M. Lazarus, Andy M Bailey, Thomas J. Simpson, Russell J. Cox*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

31 Citations (Scopus)

Abstract

The biosynthesis of the fungal metabolite tenellin from Beauveria bassiana CBS110.25 was investigated in the presence of the epigenetic modifiers 5-azacytidine and suberoyl bis-hydroxamic acid and under conditions where individual genes from the tenellin biosynthetic gene cluster were silenced. Numerous new compounds were synthesized, indicating that the normal predominant biosynthesis of tenellin is just one outcome out of a diverse array of possible products. The structures of the products reveal key clues about the programming selectivities of the tenellin polyketide synthase.

Translated title of the contributionNongenetic Reprogramming of a Fungal Highly Reducing Polyketide Synthase
Original languageEnglish
Pages (from-to)10990-10998
Number of pages9
JournalJournal of the American Chemical Society
Volume133
Issue number28
DOIs
Publication statusPublished - 20 Jul 2011

Keywords

  • FATTY-ACID SYNTHASE
  • 2-PYRIDONE TENELLIN
  • ENTOMOPATHOGENIC FUNGUS
  • STEREOCHEMISTRY
  • CLADOSPORIUM-CLADOSPORIOIDES
  • MAGNETIC-RESONANCE SPECTROSCOPY
  • LOVASTATIN BIOSYNTHESIS
  • EXPRESSION
  • BEAUVERIA-BASSIANA
  • METABOLITES

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  • Projects

    INVESTIGATING NATURAL AND NOVEL FUNGAL PKS-NRPS GENES

    Lazarus, C. M.

    1/03/071/03/10

    Project: Research

    Cite this

    Yakasai, A. A., Davison, J., Wasil, Z., Halo, L. M., Butts, C. P., Lazarus, C. M., Bailey, A. M., Simpson, T. J., & Cox, R. J. (2011). Nongenetic Reprogramming of a Fungal Highly Reducing Polyketide Synthase. Journal of the American Chemical Society, 133(28), 10990-10998. https://doi.org/10.1021/ja204200x