Nongenetic Reprogramming of a Fungal Highly Reducing Polyketide Synthase

Ahmed A. Yakasai; Jack Davison; Zahida Wasil; Laura M. Halo; Craig P. Butts; Colin M. Lazarus; Andy M Bailey; Thomas J. Simpson; Russell J. Cox

doi:10.1021/ja204200x

Nongenetic Reprogramming of a Fungal Highly Reducing Polyketide Synthase

Ahmed A. Yakasai, Jack Davison, Zahida Wasil, Laura M. Halo, Craig P. Butts, Colin M. Lazarus, Andy M Bailey, Thomas J. Simpson, Russell J. Cox^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

44 Citations (Scopus)

Abstract

The biosynthesis of the fungal metabolite tenellin from Beauveria bassiana CBS110.25 was investigated in the presence of the epigenetic modifiers 5-azacytidine and suberoyl bis-hydroxamic acid and under conditions where individual genes from the tenellin biosynthetic gene cluster were silenced. Numerous new compounds were synthesized, indicating that the normal predominant biosynthesis of tenellin is just one outcome out of a diverse array of possible products. The structures of the products reveal key clues about the programming selectivities of the tenellin polyketide synthase.

Translated title of the contribution	Nongenetic Reprogramming of a Fungal Highly Reducing Polyketide Synthase
Original language	English
Pages (from-to)	10990-10998
Number of pages	9
Journal	Journal of the American Chemical Society
Volume	133
Issue number	28
DOIs	https://doi.org/10.1021/ja204200x
Publication status	Published - 20 Jul 2011

Research Groups and Themes

Organic & Biological

Keywords

FATTY-ACID SYNTHASE
2-PYRIDONE TENELLIN
ENTOMOPATHOGENIC FUNGUS
STEREOCHEMISTRY
CLADOSPORIUM-CLADOSPORIOIDES
MAGNETIC-RESONANCE SPECTROSCOPY
LOVASTATIN BIOSYNTHESIS
EXPRESSION
BEAUVERIA-BASSIANA
METABOLITES

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10.1021/ja204200x

http://pubs.acs.org/doi/abs/10.1021/ja204200x

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@article{c5b8d04e964441be808f45b68fc33caa,

title = "Nongenetic Reprogramming of a Fungal Highly Reducing Polyketide Synthase",

abstract = "The biosynthesis of the fungal metabolite tenellin from Beauveria bassiana CBS110.25 was investigated in the presence of the epigenetic modifiers 5-azacytidine and suberoyl bis-hydroxamic acid and under conditions where individual genes from the tenellin biosynthetic gene cluster were silenced. Numerous new compounds were synthesized, indicating that the normal predominant biosynthesis of tenellin is just one outcome out of a diverse array of possible products. The structures of the products reveal key clues about the programming selectivities of the tenellin polyketide synthase.",

keywords = "FATTY-ACID SYNTHASE, 2-PYRIDONE TENELLIN, ENTOMOPATHOGENIC FUNGUS, STEREOCHEMISTRY, CLADOSPORIUM-CLADOSPORIOIDES, MAGNETIC-RESONANCE SPECTROSCOPY, LOVASTATIN BIOSYNTHESIS, EXPRESSION, BEAUVERIA-BASSIANA, METABOLITES",

author = "Yakasai, \{Ahmed A.\} and Jack Davison and Zahida Wasil and Halo, \{Laura M.\} and Butts, \{Craig P.\} and Lazarus, \{Colin M.\} and Bailey, \{Andy M\} and Simpson, \{Thomas J.\} and Cox, \{Russell J.\}",

year = "2011",

month = jul,

day = "20",

doi = "10.1021/ja204200x",

language = "English",

volume = "133",

pages = "10990--10998",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "28",

}

TY - JOUR

T1 - Nongenetic Reprogramming of a Fungal Highly Reducing Polyketide Synthase

AU - Yakasai, Ahmed A.

AU - Davison, Jack

AU - Wasil, Zahida

AU - Halo, Laura M.

AU - Butts, Craig P.

AU - Lazarus, Colin M.

AU - Bailey, Andy M

AU - Simpson, Thomas J.

AU - Cox, Russell J.

PY - 2011/7/20

Y1 - 2011/7/20

N2 - The biosynthesis of the fungal metabolite tenellin from Beauveria bassiana CBS110.25 was investigated in the presence of the epigenetic modifiers 5-azacytidine and suberoyl bis-hydroxamic acid and under conditions where individual genes from the tenellin biosynthetic gene cluster were silenced. Numerous new compounds were synthesized, indicating that the normal predominant biosynthesis of tenellin is just one outcome out of a diverse array of possible products. The structures of the products reveal key clues about the programming selectivities of the tenellin polyketide synthase.

AB - The biosynthesis of the fungal metabolite tenellin from Beauveria bassiana CBS110.25 was investigated in the presence of the epigenetic modifiers 5-azacytidine and suberoyl bis-hydroxamic acid and under conditions where individual genes from the tenellin biosynthetic gene cluster were silenced. Numerous new compounds were synthesized, indicating that the normal predominant biosynthesis of tenellin is just one outcome out of a diverse array of possible products. The structures of the products reveal key clues about the programming selectivities of the tenellin polyketide synthase.

KW - FATTY-ACID SYNTHASE

KW - 2-PYRIDONE TENELLIN

KW - ENTOMOPATHOGENIC FUNGUS

KW - STEREOCHEMISTRY

KW - CLADOSPORIUM-CLADOSPORIOIDES

KW - MAGNETIC-RESONANCE SPECTROSCOPY

KW - LOVASTATIN BIOSYNTHESIS

KW - EXPRESSION

KW - BEAUVERIA-BASSIANA

KW - METABOLITES

UR - https://www.scopus.com/pages/publications/79960206966

U2 - 10.1021/ja204200x

DO - 10.1021/ja204200x

M3 - Article (Academic Journal)

C2 - 21675761

SN - 0002-7863

VL - 133

SP - 10990

EP - 10998

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 28

ER -

Nongenetic Reprogramming of a Fungal Highly Reducing Polyketide Synthase

Abstract

Research Groups and Themes

Keywords

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INVESTIGATING NATURAL AND NOVEL FUNGAL PKS-NRPS GENES

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Nongenetic Reprogramming of a Fungal Highly Reducing Polyketide Synthase

Abstract

Research Groups and Themes

Keywords

Access to Document

Handle.net

Other files and links

Fingerprint

Projects

INVESTIGATING NATURAL AND NOVEL FUNGAL PKS-NRPS GENES

Cite this