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On the complex point spread function in interferometric cross-polarisation microscopy

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)1232-1239
Number of pages8
JournalOptics Express
Volume23
Issue number2
DOIs
DatePublished - 20 Jan 2015

Abstract

The ability to characterize the Point Spread Function(PSF) is crucial in practical microscopy, but requires knowledge of the complex PSF for approaches that detect fields instead of intensities. Here we experimentally measure and theoretically model the volumetric amplitude and phase response of an Interferometric Cross-polarisation Microscope to demonstrate the technique’s capability to provide confocal-like images of weakly birefringent structures in living cells. We find the axial FWHM of the amplitude PSF to be 0.70 ± 0.01 μm and 0.83 μm for model and measurement, respectively, on par with confocal microscopy. Ultimately retaining both amplitude and phase information will however enable approaches for improved localisation of objects.

    Research areas

  • Confocal Microscopy, Interference , Scattering, Point Spread Function, Polarization, Sectioning

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  • AxialFocusMeasurement__sb2_v2

    Rights statement: This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-23-2-1232. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

    Accepted author manuscript, 1 MB, PDF document

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