On the sensitivity of Interferometric Cross-Polarisation Microscopy for nanoparticle detection in the near-infrared.

Ben Miles; Elizabeth Robinson; Erik  MPH van Dijk; Ian Lindsay; Niek F van Hulst; Henkjan Gersen

doi:10.1021/acsphotonics.5b00326

On the sensitivity of Interferometric Cross-Polarisation Microscopy for nanoparticle detection in the near-infrared.

Ben Miles, Elizabeth Robinson, Erik MPH van Dijk, Ian Lindsay, Niek F van Hulst, Henkjan Gersen

Research output: Contribution to journal › Article (Academic Journal) › peer-review

9 Citations (Scopus)

369 Downloads (Pure)

Abstract

We address the sensitivity of Interferometric Cross-Polarisation Microscopy by comparing scattering and absorption by spherical 10nm nanoparticles through a combination of modelling and experiment. We show that orthogonality of light in the two polarisation branches of Cross-Polarisation Microscopy ensures that only light that has interacted with a nanoparticle is interferometrically enhanced. As a result background-free shot noise-limited detection is achieved for sub-μW optical powers at the sample. Our modelling in particular shows that in the near-infrared regime, above the plasmon resonance frequency of spherical nanoparticles, the cross-polarisation approach is several orders of magnitude more sensitive than conventional extinction based detection. This enhanced near-infrared sensitivity for spherical nanoparticles is promising for applications requiring low absorption and low power imaging of nanoparticles in cells.

Original language	English
Pages (from-to)	1705-1711
Number of pages	7
Journal	ACS Photonics
Volume	2
Issue number	12
Early online date	3 Nov 2015
DOIs	https://doi.org/10.1021/acsphotonics.5b00326
Publication status	Published - 17 Nov 2015

Keywords

Confocal Microscopy
Nanoparticle detection
Mie Scattering
Cross-Polarisation Imaging
Gold nanoparticles
Background free detection
Detection Sensitivity

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9 Citations
6 Article (Academic Journal)

Determining Nanorod Dimensions in Dispersion with Size Anisotropy Nanoparticle Tracking Analysis
Hoffmann, W. H., Gao, B., Mulkerns, N., Hinton, A. G., Hanna, S., Hall, S. R. & Gersen, H., 18 May 2022, In: Physical Chemistry Chemical Physics. 24, 21, p. 13040-13048 9 p.
Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access
File
2 Citations (Scopus)

64 Downloads (Pure)
On the impact of realistic point sources in spatial mode demultiplexing super resolution imaging
Greenwood, A. B., Oulton, R. & Gersen, H., 7 Nov 2022, (Accepted/In press) In: Quantum Science and Technology. 8, 1, 015024.
Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access
3 Citations (Scopus)
Direct evidence of lack of colocalisation of fluorescently labelled gold labels used in correlative light electron microscopy
Miles, B. T., Greenwood, A. B., Benito-Alifonso, D., Tanner, H., Galan, M. C., Verkade, P. & Gersen, H., 20 Mar 2017, In: Scientific Reports. 7, 7 p., 44666.
Research output: Contribution to journal › Article (Academic Journal) › peer-review

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13 Citations (Scopus)

364 Downloads (Pure)

2 Finished

ULTRASENSITIVE DETECTION OF NANOMETER SIZED METAL PARTICLES IN LIVING CELLS
Gersen, H. (Principal Investigator)
1/05/08 → 1/05/11
Project: Research
NANOSCALE CHEMICAL IMAGING: TOOLS AND TECHNIQUES FOR LOCALISED INFRARED SPECTROSCOPY OF NANOSTRUCTURED POLYMERS AND BIOMATERIALS
Lindsay, I. D. (Principal Investigator)
1/10/07 → 1/04/13
Project: Research

Dr Henkjan Gersen
- H.Gersenbristol.acuk
- School of Physics - Honorary Industrial Professor
Person: Honorary and Visiting Academic

Cite this

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title = "On the sensitivity of Interferometric Cross-Polarisation Microscopy for nanoparticle detection in the near-infrared.",

abstract = "We address the sensitivity of Interferometric Cross-Polarisation Microscopy by comparing scattering and absorption by spherical 10nm nanoparticles through a combination of modelling and experiment. We show that orthogonality of light in the two polarisation branches of Cross-Polarisation Microscopy ensures that only light that has interacted with a nanoparticle is interferometrically enhanced. As a result background-free shot noise-limited detection is achieved for sub-μW optical powers at the sample. Our modelling in particular shows that in the near-infrared regime, above the plasmon resonance frequency of spherical nanoparticles, the cross-polarisation approach is several orders of magnitude more sensitive than conventional extinction based detection. This enhanced near-infrared sensitivity for spherical nanoparticles is promising for applications requiring low absorption and low power imaging of nanoparticles in cells.",

keywords = "Confocal Microscopy, Nanoparticle detection, Mie Scattering, Cross-Polarisation Imaging, Gold nanoparticles, Background free detection, Detection Sensitivity",

author = "Ben Miles and Elizabeth Robinson and {van Dijk}, {Erik MPH} and Ian Lindsay and {van Hulst}, {Niek F} and Henkjan Gersen",

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doi = "10.1021/acsphotonics.5b00326",

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AU - Miles, Ben

AU - Robinson, Elizabeth

AU - van Dijk, Erik MPH

AU - Lindsay, Ian

AU - van Hulst, Niek F

AU - Gersen, Henkjan

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AB - We address the sensitivity of Interferometric Cross-Polarisation Microscopy by comparing scattering and absorption by spherical 10nm nanoparticles through a combination of modelling and experiment. We show that orthogonality of light in the two polarisation branches of Cross-Polarisation Microscopy ensures that only light that has interacted with a nanoparticle is interferometrically enhanced. As a result background-free shot noise-limited detection is achieved for sub-μW optical powers at the sample. Our modelling in particular shows that in the near-infrared regime, above the plasmon resonance frequency of spherical nanoparticles, the cross-polarisation approach is several orders of magnitude more sensitive than conventional extinction based detection. This enhanced near-infrared sensitivity for spherical nanoparticles is promising for applications requiring low absorption and low power imaging of nanoparticles in cells.

KW - Confocal Microscopy

KW - Nanoparticle detection

KW - Mie Scattering

KW - Cross-Polarisation Imaging

KW - Gold nanoparticles

KW - Background free detection

KW - Detection Sensitivity

U2 - 10.1021/acsphotonics.5b00326

DO - 10.1021/acsphotonics.5b00326

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On the sensitivity of Interferometric Cross-Polarisation Microscopy for nanoparticle detection in the near-infrared.

Abstract

Keywords

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Research output

Determining Nanorod Dimensions in Dispersion with Size Anisotropy Nanoparticle Tracking Analysis

On the impact of realistic point sources in spatial mode demultiplexing super resolution imaging

Direct evidence of lack of colocalisation of fluorescently labelled gold labels used in correlative light electron microscopy

Projects

ULTRASENSITIVE DETECTION OF NANOMETER SIZED METAL PARTICLES IN LIVING CELLS

NANOSCALE CHEMICAL IMAGING: TOOLS AND TECHNIQUES FOR LOCALISED INFRARED SPECTROSCOPY OF NANOSTRUCTURED POLYMERS AND BIOMATERIALS

Profiles

Dr Henkjan Gersen

Cite this