Optical single-channel resolution imaging of the ryanodine receptor distribution in rat cardiac myocytes

D Baddeley; I D Jayasinghe; L Lam; S Rossberger; MB Cannell; C Soeller

doi:10.1073/pnas.0908971106

Optical single-channel resolution imaging of the ryanodine receptor distribution in rat cardiac myocytes

D Baddeley, I D Jayasinghe, L Lam, S Rossberger, MB Cannell, C Soeller

School of Physiology, Pharmacology & Neuroscience

Research output: Contribution to journal › Article (Academic Journal) › peer-review

253 Citations (Scopus)

Abstract

We have applied an optical super-resolution technique based on single-molecule localization to examine the peripheral distribution of a cardiac signaling protein, the ryanodine receptor (RyR), in rat ventricular myocytes. RyRs form clusters with a mean size of approximately 14 RyRs per cluster, which is almost an order of magnitude smaller than previously estimated. Clusters were typically not circular (as previously assumed) but elongated with an average aspect ratio of 1.9. Edge-to-edge distances between adjacent RyR clusters were often

Translated title of the contribution	Optical single-channel resolution imaging of the ryanodine receptor distribution in rat cardiac myocytes
Original language	English
Pages (from-to)	22275 - 22280
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	106
Issue number	52
DOIs	https://doi.org/10.1073/pnas.0908971106
Publication status	Published - Dec 2009

Access to Document

10.1073/pnas.0908971106

http://www.pnas.org/content/106/52/22275

Handle.net

Persistent link

Cite this

@article{a895684075a7444f84e20176dfd1a17b,

title = "Optical single-channel resolution imaging of the ryanodine receptor distribution in rat cardiac myocytes",

abstract = "We have applied an optical super-resolution technique based on single-molecule localization to examine the peripheral distribution of a cardiac signaling protein, the ryanodine receptor (RyR), in rat ventricular myocytes. RyRs form clusters with a mean size of approximately 14 RyRs per cluster, which is almost an order of magnitude smaller than previously estimated. Clusters were typically not circular (as previously assumed) but elongated with an average aspect ratio of 1.9. Edge-to-edge distances between adjacent RyR clusters were often ",

author = "D Baddeley and Jayasinghe, \{I D\} and L Lam and S Rossberger and MB Cannell and C Soeller",

year = "2009",

month = dec,

doi = "10.1073/pnas.0908971106",

language = "English",

volume = "106",

pages = "22275 -- 22280",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "52",

}

TY - JOUR

T1 - Optical single-channel resolution imaging of the ryanodine receptor distribution in rat cardiac myocytes

AU - Baddeley, D

AU - Jayasinghe, I D

AU - Lam, L

AU - Rossberger, S

AU - Cannell, MB

AU - Soeller, C

PY - 2009/12

Y1 - 2009/12

N2 - We have applied an optical super-resolution technique based on single-molecule localization to examine the peripheral distribution of a cardiac signaling protein, the ryanodine receptor (RyR), in rat ventricular myocytes. RyRs form clusters with a mean size of approximately 14 RyRs per cluster, which is almost an order of magnitude smaller than previously estimated. Clusters were typically not circular (as previously assumed) but elongated with an average aspect ratio of 1.9. Edge-to-edge distances between adjacent RyR clusters were often

AB - We have applied an optical super-resolution technique based on single-molecule localization to examine the peripheral distribution of a cardiac signaling protein, the ryanodine receptor (RyR), in rat ventricular myocytes. RyRs form clusters with a mean size of approximately 14 RyRs per cluster, which is almost an order of magnitude smaller than previously estimated. Clusters were typically not circular (as previously assumed) but elongated with an average aspect ratio of 1.9. Edge-to-edge distances between adjacent RyR clusters were often

UR - https://www.scopus.com/pages/publications/76049092344

U2 - 10.1073/pnas.0908971106

DO - 10.1073/pnas.0908971106

M3 - Article (Academic Journal)

C2 - 20018773

SN - 0027-8424

VL - 106

SP - 22275

EP - 22280

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 52

ER -

Optical single-channel resolution imaging of the ryanodine receptor distribution in rat cardiac myocytes

Abstract

Access to Document

Handle.net

Other files and links

Fingerprint

Cite this