Optical techniques and microtools for subcellular delivery and sampling

Duncan Casey*, Douglas Wylie, Mark Neil

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

Abstract

The vast majority of biochemical and histological assays are conducted upon ensembles of tens or hundreds or thousands of cells. However, there is a robust and increasing volume of evidence that small subpopulations of cells play significant roles in the development of many disease states, and particularly in their evolution toward treatment resistance. As such, a novel suite of optical tools has been and is being developed, offering functionality analogous to traditional techniques but furnishing them with single- or even subcellular resolution. These are designed to probe the operations of life at its most fundamental level, providing insights into the complex, crowded, and seemingly chaotic processes underpinning cellular processes and their malfunctions.In this chapter, the development of these tools will be explored from their origins to their current state of the art, alongside their applications in both proof-of-concept and applied clinical roles. The focus will be upon tools and techniques designed and developed to interact directly with the cell membrane, taking in optical lysis, poration, and fusion techniques alongside surface chemistry and thin-film coatings capable of causing structural effects within a target cell's plasma membrane. Notable successes and problems will be critically analyzed, and the prospects for the future will be discussed with reference to emerging technologies and promising research avenues in the field.

Original languageEnglish
Title of host publicationLight Robotics-Structure-Mediated Nanobiophotonics
PublisherElsevier Inc.
Pages287-311
Number of pages25
ISBN (Electronic)9780081022481
ISBN (Print)9780702070969
DOIs
Publication statusPublished - 30 May 2017

Keywords

  • Microtools
  • Optical lysis
  • Plasma membrane
  • Poration
  • Subcellular
  • Thin-film coatings

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    Casey, D., Wylie, D., & Neil, M. (2017). Optical techniques and microtools for subcellular delivery and sampling. In Light Robotics-Structure-Mediated Nanobiophotonics (pp. 287-311). Elsevier Inc.. https://doi.org/10.1016/B978-0-7020-7096-9.00010-0