Optical Tools for Single-Cell Manipulation and Analysis

Duncan Casey; Jayne Dooley

doi:10.1007/978-3-662-49118-8

Optical Tools for Single-Cell Manipulation and Analysis

Duncan Casey
, Jayne Dooley

School of Physics

Research output: Chapter in Book/Report/Conference proceeding › Chapter in a book

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Abstract

Experiments on individual cells require a range of extremely precise tools to permit their selection, manipulation, stimulation and analysis. This is further complicated by the cells’ sensitivity to their environment, meaning that such tools must also be very gentle (or at least very localised) to minimise the generation of artefacts. Optical tools provide ideal performance in a number of such roles, exhibiting high spatial and temporal selectivity while causing minimal non-specific effects. This chapter focuses upon the optical tools that have been developed for these purposes, ranging from optical trapping systems which provide a contact-free technique for the manipulation of micron-scale objects, through to a selection of the different optically mediated cell membrane disruption methods available for lysis and/or delivery of material.

Original language	English
Title of host publication	Essentials of Single-Cell Analysis
Subtitle of host publication	Concepts, Applications and Future Prospects
Editors	Fan-Gang Tseng, Tuhin Santra
Publisher	Springer Berlin Heidelberg
Chapter	5
Pages	131-57
Number of pages	26
Edition	1
ISBN (Electronic)	978-3-662-49118-8
ISBN (Print)	978-3-662-49116-4, 978-3-662-56980-1
DOIs	https://doi.org/10.1007/978-3-662-49118-8 https://doi.org/10.1007/978-3-662-49118-8_5
Publication status	Published - 22 Jan 2016

Publication series

Name	Series in BioEngineering
Publisher	Springer
ISSN (Print)	2196-8861
ISSN (Electronic)	2196-887X

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Optical Tools for Single-Cell Manipulation and Analysis

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