Osteogenic and bactericidal surfaces from hydrothermal titania nanowires on titanium substrates

Penelope M Tsimbouri, Leanne Fisher, N Holloway, Terje Sjostrom, Angela Nobbs, Dominic Meek, Bo Su, Matthew J. Dalby

Research output: Contribution to journalArticle (Academic Journal)peer-review

90 Citations (Scopus)
361 Downloads (Pure)


Nanotopographical cues on Ti have been shown to elicit different cell responses such as cell differentiation and selective growth. Bone remodelling is a constant process requiring specific cues for optimal bone growth and implant fixation. Moreover, biofilm formation and the resulting infection on surgical implants is a major issue. Our aim is to identify nanopatterns on Ti surfaces that would be optimal for both bone remodelling and for reducing risk of bacterial infection. Primary human osteoblast/osteoclast co-cultures were seeded onto Ti substrates with TiO2 nanowires grown under alkaline conditions at 240°C for different times (2, 2.5 or 3h). Cell growth and behaviour was assessed by scanning electron microscopy (SEM), immunofluorescence microscopy, histochemistry and quantitative RT-PCR methods. Bacterial colonisation of the nanowire surfaces was also assessed by confocal microscopy and SEM. From the three surfaces tested the 2h nanowire surface supported osteoblast and to a lesser extent osteoclast growth and differentiation. At the same time bacterial viability was reduced. Hence the 2h surface provided optimal bone remodeling in vitro conditions while reducing infection risk, making it a favourable candidate for future implant surfaces.
Original languageEnglish
Article number36857
Number of pages12
JournalScientific Reports
Publication statusPublished - 18 Nov 2016


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