Overcoming cloning problems by staining agarose gels with crystal violet instead of ethidium bromide in lactate dehydrogenase gene from Plasmodium vivax and Plasmodium falciparum

D. Turgut-Balik*, V. Çelik, Kath Moreton, R. L. Brady

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

4 Citations (Scopus)

Abstract

In this study, lactate dehydrogenase gene from Plasmodium vivax has been tried to subclone into an expression vector. Some of the Plasmodium falciparum lactate dehydrogenase mutant genes have also been tried to clone and subclone into a vector, but we failed to clone or subclone either of the genes. DNA visualisation in electrophoretic gels typically requires UV radiation and the fluorecent dye ethidium bromide. A crystal violet-stained gel was run instead of an ethidium bromide gel and so avoided the use of UV radiation. This enabled us to clone or subclone both Plasmodium vivax lactate dehydrogenase gene and Plasmodium falciparum lactate dehydrogenase mutant genes into any desired vector.

Original languageEnglish
Pages (from-to)389-397
Number of pages9
JournalActa Biologica Hungarica
Volume56
Issue number3-4
DOIs
Publication statusPublished - 12 Sep 2005

Keywords

  • Crystal violet
  • Ethidium bromide
  • Lactate dehydrogenase
  • Plasmodium falciparum
  • Plasmodium vivax

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