TY - JOUR
T1 - Overexpression of a plant reticulon remodels the lumen of the cortical endoplasmic reticulum but does not perturb protein transport
AU - Tolley, Nicholas
AU - Sparkes, Imogen A
AU - Hunter, Paul R
AU - Craddock, Christian P
AU - Nuttall, James
AU - Roberts, Lynne M
AU - Hawes, Chris
AU - Pedrazzini, Emanuela
AU - Frigerio, Lorenzo
PY - 2008/1
Y1 - 2008/1
N2 - We have cloned a member of the reticulon (RTN) family of Arabidopsis thaliana (RTNLB13). When fused to yellow fluorescent protein (YFP) and expressed in tobacco leaf epidermal cells, RTNLB13 is localized in the endoplasmic reticulum (ER). Coexpression of a soluble ER luminal marker reveals that YFP-tagged, myc-tagged or untagged RTNLB13 induces severe morphological changes to the lumen of the ER. We show, using fluorescence recovery after photobleaching (FRAP) analysis, that RTNLB13 overexpression greatly reduces diffusion of soluble proteins within the ER lumen, possibly by introducing constrictions into the membrane. In spite of this severe phenotype, Golgi shape, number and dynamics appear unperturbed and secretion of a reporter protein remains unaffected.
AB - We have cloned a member of the reticulon (RTN) family of Arabidopsis thaliana (RTNLB13). When fused to yellow fluorescent protein (YFP) and expressed in tobacco leaf epidermal cells, RTNLB13 is localized in the endoplasmic reticulum (ER). Coexpression of a soluble ER luminal marker reveals that YFP-tagged, myc-tagged or untagged RTNLB13 induces severe morphological changes to the lumen of the ER. We show, using fluorescence recovery after photobleaching (FRAP) analysis, that RTNLB13 overexpression greatly reduces diffusion of soluble proteins within the ER lumen, possibly by introducing constrictions into the membrane. In spite of this severe phenotype, Golgi shape, number and dynamics appear unperturbed and secretion of a reporter protein remains unaffected.
KW - Arabidopsis
KW - Arabidopsis Proteins
KW - Endoplasmic Reticulum
KW - Fluorescence Recovery After Photobleaching
KW - Golgi Apparatus
KW - Microscopy, Confocal
KW - Phylogeny
KW - Protein Transport
KW - Recombinant Proteins
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1111/j.1600-0854.2007.00670.x
DO - 10.1111/j.1600-0854.2007.00670.x
M3 - Article (Academic Journal)
C2 - 17980018
SN - 1398-9219
VL - 9
SP - 94
EP - 102
JO - Traffic
JF - Traffic
IS - 1
ER -