Overexpression of a plant reticulon remodels the lumen of the cortical endoplasmic reticulum but does not perturb protein transport

Nicholas Tolley, Imogen A Sparkes, Paul R Hunter, Christian P Craddock, James Nuttall, Lynne M Roberts, Chris Hawes, Emanuela Pedrazzini, Lorenzo Frigerio

Research output: Contribution to journalArticle (Academic Journal)peer-review

96 Citations (Scopus)

Abstract

We have cloned a member of the reticulon (RTN) family of Arabidopsis thaliana (RTNLB13). When fused to yellow fluorescent protein (YFP) and expressed in tobacco leaf epidermal cells, RTNLB13 is localized in the endoplasmic reticulum (ER). Coexpression of a soluble ER luminal marker reveals that YFP-tagged, myc-tagged or untagged RTNLB13 induces severe morphological changes to the lumen of the ER. We show, using fluorescence recovery after photobleaching (FRAP) analysis, that RTNLB13 overexpression greatly reduces diffusion of soluble proteins within the ER lumen, possibly by introducing constrictions into the membrane. In spite of this severe phenotype, Golgi shape, number and dynamics appear unperturbed and secretion of a reporter protein remains unaffected.

Original languageEnglish
Pages (from-to)94-102
Number of pages9
JournalTraffic
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 2008

Keywords

  • Arabidopsis
  • Arabidopsis Proteins
  • Endoplasmic Reticulum
  • Fluorescence Recovery After Photobleaching
  • Golgi Apparatus
  • Microscopy, Confocal
  • Phylogeny
  • Protein Transport
  • Recombinant Proteins
  • Journal Article
  • Research Support, Non-U.S. Gov't

Fingerprint Dive into the research topics of 'Overexpression of a plant reticulon remodels the lumen of the cortical endoplasmic reticulum but does not perturb protein transport'. Together they form a unique fingerprint.

Cite this