Overexpression of scavenger receptor LOX-1 in endothelial cells promotes atherogenesis in the ApoE−/− mouse model

SJ White, GB Sala-Newby, AC Newby

Research output: Contribution to journalArticle (Academic Journal)peer-review

28 Citations (Scopus)

Abstract

Abstract Aims The oxidized low-density lipoprotein receptor LOX-1 is up-regulated on activated endothelial cells, for example, the endothelium of atherosclerosis-prone sites, in both human and animal models. We examined whether endothelial LOX-1 overexpression may contribute to atherogenesis. Methods Adenoviral vectors expressing LOX-1 or LOXIN (a splice variant of LOX-1 with inhibitory function) were created and used to transduce the normally lesion-free common carotid artery, in high fat-fed female ApoE−/− mice. Mice were placed on high-fat diet for 4 weeks prior to gene transfer with either LOX-1 or a combination of LOX-1 and LOXIN, and assessment of plaque development analyzed 6 weeks following gene transfer. Results Compared to controls, LOX-1 transduction induced a significant increase in plaque coverage within the common carotid artery to 91% compared to 50% after RAd66 control virus infection (P≤.05). This was inhibited by co-expression of LOXIN (62%). Conclusions These results demonstrate that up-regulation of LOX-1 promotes atherogenesis, highlighting LOX-1 function as a target for intervention. In addition, this study further demonstrated the inhibitory function of LOXIN.
Translated title of the contributionOverexpression of scavenger receptor LOX-1 in endothelial cells promotes atherogenesis in the ApoE−/− mouse model
Original languageEnglish
Pages (from-to)369 - 373
Number of pages5
JournalCardiovascular Pathology
Volume20 (6)
DOIs
Publication statusPublished - Nov 2011

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