Pam3CSK4 and LTA-TLRs Ligands Associated with Microdomains Induce IL8 Production in Human Adrenocortical Cancer Cells

W Kanczkowski; H Morawietz; CG Ziegler; RH Funk; G Schmitz; KD Zacharowski; CE Mohn; M Ehrhart-Bornstein; SR Bornstein

doi:10.1055/s-2007-980189

Pam3CSK4 and LTA-TLRs Ligands Associated with Microdomains Induce IL8 Production in Human Adrenocortical Cancer Cells

W Kanczkowski, H Morawietz, CG Ziegler, RH Funk, G Schmitz, KD Zacharowski, CE Mohn, M Ehrhart-Bornstein, SR Bornstein

Research output: Contribution to journal › Article (Academic Journal) › peer-review

14 Citations (Scopus)

Abstract

Bacterially derived ligands, Pam (3)CSK (4) and LPS, can directly impact adrenal glands steroidogenesis through microdomain-related TLR1/2 and 4, respectively, and indirectly via immune cell-derived cytokines. The bilateral immunoadrenal relationship plays an important role in the proper functioning of both systems. CXC chemokine-dependent immune cell infiltration into adrenocortical carcinomas (ACC), which correlates with poor prognosis, is a common phenomenon. Recently, IL8 was identified in ACC and NCI-H295R cells, and was found to contribute to ACC tumour growth. The aim of this study was to clarify the role of different TLR ligands in IL8 production in NCI-H295R cells. This is the first study to demonstrate the expression of several TLRs including TLR1, 3, 6, 7 and 9 in human adrenocortical cells by using the RT-PCR approach. Only stimulation with TLR1/6 together with TLR2 ligands resulted in IL8 peptide and mRNA induction in a dose and time-dependent manner. Our data suggest that gram-positive bacteria-related TLR1/2/6 ligands might contribute to adrenal gland tumorigenesis via IL8 production.

Translated title of the contribution	Pam3CSK4 and LTA-TLRs Ligands Associated with Microdomains Induce IL8 Production in Human Adrenocortical Cancer Cells
Original language	English
Pages (from-to)	457 - 460
Number of pages	4
Journal	Hormone and Metabolic Research
Volume	39 (6)
DOIs	https://doi.org/10.1055/s-2007-980189
Publication status	Published - Jun 2007

Bibliographical note

Publisher: Thieme

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@article{f643494c21344f46a148f77559f569b4,

title = "Pam3CSK4 and LTA-TLRs Ligands Associated with Microdomains Induce IL8 Production in Human Adrenocortical Cancer Cells",

abstract = "Bacterially derived ligands, Pam (3)CSK (4) and LPS, can directly impact adrenal glands steroidogenesis through microdomain-related TLR1/2 and 4, respectively, and indirectly via immune cell-derived cytokines. The bilateral immunoadrenal relationship plays an important role in the proper functioning of both systems. CXC chemokine-dependent immune cell infiltration into adrenocortical carcinomas (ACC), which correlates with poor prognosis, is a common phenomenon. Recently, IL8 was identified in ACC and NCI-H295R cells, and was found to contribute to ACC tumour growth. The aim of this study was to clarify the role of different TLR ligands in IL8 production in NCI-H295R cells. This is the first study to demonstrate the expression of several TLRs including TLR1, 3, 6, 7 and 9 in human adrenocortical cells by using the RT-PCR approach. Only stimulation with TLR1/6 together with TLR2 ligands resulted in IL8 peptide and mRNA induction in a dose and time-dependent manner. Our data suggest that gram-positive bacteria-related TLR1/2/6 ligands might contribute to adrenal gland tumorigenesis via IL8 production.",

author = "W Kanczkowski and H Morawietz and CG Ziegler and RH Funk and G Schmitz and KD Zacharowski and CE Mohn and M Ehrhart-Bornstein and SR Bornstein",

note = "Publisher: Thieme",

year = "2007",

month = jun,

doi = "10.1055/s-2007-980189",

language = "English",

volume = "39 (6)",

pages = "457 -- 460",

journal = "Hormone and Metabolic Research",

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}

Pam3CSK4 and LTA-TLRs Ligands Associated with Microdomains Induce IL8 Production in Human Adrenocortical Cancer Cells. / Kanczkowski, W; Morawietz, H; Ziegler, CG; Funk, RH; Schmitz, G; Zacharowski, KD; Mohn, CE; Ehrhart-Bornstein, M; Bornstein, SR.

In: Hormone and Metabolic Research, Vol. 39 (6), 06.2007, p. 457 - 460.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

TY - JOUR

T1 - Pam3CSK4 and LTA-TLRs Ligands Associated with Microdomains Induce IL8 Production in Human Adrenocortical Cancer Cells

AU - Kanczkowski, W

AU - Morawietz, H

AU - Ziegler, CG

AU - Funk, RH

AU - Schmitz, G

AU - Zacharowski, KD

AU - Mohn, CE

AU - Ehrhart-Bornstein, M

AU - Bornstein, SR

N1 - Publisher: Thieme

PY - 2007/6

Y1 - 2007/6

N2 - Bacterially derived ligands, Pam (3)CSK (4) and LPS, can directly impact adrenal glands steroidogenesis through microdomain-related TLR1/2 and 4, respectively, and indirectly via immune cell-derived cytokines. The bilateral immunoadrenal relationship plays an important role in the proper functioning of both systems. CXC chemokine-dependent immune cell infiltration into adrenocortical carcinomas (ACC), which correlates with poor prognosis, is a common phenomenon. Recently, IL8 was identified in ACC and NCI-H295R cells, and was found to contribute to ACC tumour growth. The aim of this study was to clarify the role of different TLR ligands in IL8 production in NCI-H295R cells. This is the first study to demonstrate the expression of several TLRs including TLR1, 3, 6, 7 and 9 in human adrenocortical cells by using the RT-PCR approach. Only stimulation with TLR1/6 together with TLR2 ligands resulted in IL8 peptide and mRNA induction in a dose and time-dependent manner. Our data suggest that gram-positive bacteria-related TLR1/2/6 ligands might contribute to adrenal gland tumorigenesis via IL8 production.

AB - Bacterially derived ligands, Pam (3)CSK (4) and LPS, can directly impact adrenal glands steroidogenesis through microdomain-related TLR1/2 and 4, respectively, and indirectly via immune cell-derived cytokines. The bilateral immunoadrenal relationship plays an important role in the proper functioning of both systems. CXC chemokine-dependent immune cell infiltration into adrenocortical carcinomas (ACC), which correlates with poor prognosis, is a common phenomenon. Recently, IL8 was identified in ACC and NCI-H295R cells, and was found to contribute to ACC tumour growth. The aim of this study was to clarify the role of different TLR ligands in IL8 production in NCI-H295R cells. This is the first study to demonstrate the expression of several TLRs including TLR1, 3, 6, 7 and 9 in human adrenocortical cells by using the RT-PCR approach. Only stimulation with TLR1/6 together with TLR2 ligands resulted in IL8 peptide and mRNA induction in a dose and time-dependent manner. Our data suggest that gram-positive bacteria-related TLR1/2/6 ligands might contribute to adrenal gland tumorigenesis via IL8 production.

U2 - 10.1055/s-2007-980189

DO - 10.1055/s-2007-980189

M3 - Article (Academic Journal)

C2 - 17578764

VL - 39 (6)

SP - 457

EP - 460

JO - Hormone and Metabolic Research

JF - Hormone and Metabolic Research

SN - 0018-5043

ER -