Pam3CSK4 and LTA-TLRs Ligands Associated with Microdomains Induce IL8 Production in Human Adrenocortical Cancer Cells

W Kanczkowski, H Morawietz, CG Ziegler, RH Funk, G Schmitz, KD Zacharowski, CE Mohn, M Ehrhart-Bornstein, SR Bornstein

    Research output: Contribution to journalArticle (Academic Journal)peer-review

    14 Citations (Scopus)

    Abstract

    Bacterially derived ligands, Pam (3)CSK (4) and LPS, can directly impact adrenal glands steroidogenesis through microdomain-related TLR1/2 and 4, respectively, and indirectly via immune cell-derived cytokines. The bilateral immunoadrenal relationship plays an important role in the proper functioning of both systems. CXC chemokine-dependent immune cell infiltration into adrenocortical carcinomas (ACC), which correlates with poor prognosis, is a common phenomenon. Recently, IL8 was identified in ACC and NCI-H295R cells, and was found to contribute to ACC tumour growth. The aim of this study was to clarify the role of different TLR ligands in IL8 production in NCI-H295R cells. This is the first study to demonstrate the expression of several TLRs including TLR1, 3, 6, 7 and 9 in human adrenocortical cells by using the RT-PCR approach. Only stimulation with TLR1/6 together with TLR2 ligands resulted in IL8 peptide and mRNA induction in a dose and time-dependent manner. Our data suggest that gram-positive bacteria-related TLR1/2/6 ligands might contribute to adrenal gland tumorigenesis via IL8 production.
    Translated title of the contributionPam3CSK4 and LTA-TLRs Ligands Associated with Microdomains Induce IL8 Production in Human Adrenocortical Cancer Cells
    Original languageEnglish
    Pages (from-to)457 - 460
    Number of pages4
    JournalHormone and Metabolic Research
    Volume39 (6)
    DOIs
    Publication statusPublished - Jun 2007

    Bibliographical note

    Publisher: Thieme

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