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Parkin-mediated ubiquitination contributes to the constitutive turnover of mitochondrial fission factor (Mff)

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Parkin-mediated ubiquitination contributes to the constitutive turnover of mitochondrial fission factor (Mff). / Lee, Laura; Seager, Richard; Nakamura, Yasuko; Wilkinson, Kevin; Henley, Jeremy.

In: PLoS ONE, Vol. 14, No. 5, e0213116, 21.05.2019.

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@article{27ee47560b7345d493125deba88adfcb,
title = "Parkin-mediated ubiquitination contributes to the constitutive turnover of mitochondrial fission factor (Mff)",
abstract = "The mitochondrial outer membrane protein Mitochondrial Fission Factor (Mff) plays a key role in both physiological and pathological fission. It is well established that in stressed or functionally impaired mitochondria the PINK1 recruits the ubiquitin ligase Parkin which ubiquitinates Mff to facilitate the removal of defective mitochondria and maintain the integrity mitochondrial network. Here we show that, in addition to this clearance pathway, Parkin also ubiquitinates Mff in a PINK1-dependent manner under basal, non-stressed conditions to regulate constitutive Mff turnover. We further show that removing Parkin with shRNA knockdown does not completely prevent Mff ubiquitination under these conditions indicating that at least one other ubiquitin ligase contributes to Mff proteostasis. These data demonstrate that Parkin plays a role in physiological maintenance of mitochondrial membrane protein composition in healthy mitochondria through constitutive low-level activation.",
author = "Laura Lee and Richard Seager and Yasuko Nakamura and Kevin Wilkinson and Jeremy Henley",
year = "2019",
month = "5",
day = "21",
doi = "10.1371/journal.pone.0213116",
language = "English",
volume = "14",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

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TY - JOUR

T1 - Parkin-mediated ubiquitination contributes to the constitutive turnover of mitochondrial fission factor (Mff)

AU - Lee, Laura

AU - Seager, Richard

AU - Nakamura, Yasuko

AU - Wilkinson, Kevin

AU - Henley, Jeremy

PY - 2019/5/21

Y1 - 2019/5/21

N2 - The mitochondrial outer membrane protein Mitochondrial Fission Factor (Mff) plays a key role in both physiological and pathological fission. It is well established that in stressed or functionally impaired mitochondria the PINK1 recruits the ubiquitin ligase Parkin which ubiquitinates Mff to facilitate the removal of defective mitochondria and maintain the integrity mitochondrial network. Here we show that, in addition to this clearance pathway, Parkin also ubiquitinates Mff in a PINK1-dependent manner under basal, non-stressed conditions to regulate constitutive Mff turnover. We further show that removing Parkin with shRNA knockdown does not completely prevent Mff ubiquitination under these conditions indicating that at least one other ubiquitin ligase contributes to Mff proteostasis. These data demonstrate that Parkin plays a role in physiological maintenance of mitochondrial membrane protein composition in healthy mitochondria through constitutive low-level activation.

AB - The mitochondrial outer membrane protein Mitochondrial Fission Factor (Mff) plays a key role in both physiological and pathological fission. It is well established that in stressed or functionally impaired mitochondria the PINK1 recruits the ubiquitin ligase Parkin which ubiquitinates Mff to facilitate the removal of defective mitochondria and maintain the integrity mitochondrial network. Here we show that, in addition to this clearance pathway, Parkin also ubiquitinates Mff in a PINK1-dependent manner under basal, non-stressed conditions to regulate constitutive Mff turnover. We further show that removing Parkin with shRNA knockdown does not completely prevent Mff ubiquitination under these conditions indicating that at least one other ubiquitin ligase contributes to Mff proteostasis. These data demonstrate that Parkin plays a role in physiological maintenance of mitochondrial membrane protein composition in healthy mitochondria through constitutive low-level activation.

UR - http://www.scopus.com/inward/record.url?scp=85065961430&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0213116

DO - 10.1371/journal.pone.0213116

M3 - Article

C2 - 31112535

AN - SCOPUS:85065961430

VL - 14

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 5

M1 - e0213116

ER -