Patterned growth of neuronal cells on modified diamond-like carbon substrates

S Kelly, EM Regan, JB Uney, AD Dick, JP McGeehan, Group The Bristol Biochip, EJ Mayer, F Claeyssens

Research output: Contribution to journalArticle (Academic Journal)peer-review

56 Citations (Scopus)

Abstract

Diamond-like carbon (DLC) has been explored as a biomaterial with potential use for coating implantable devices and surgical instruments. In this study the interaction of DLC with mammalian neuronal cells has been studied along with its modifications to improve its function as a biomaterial. We describe the use of DLC, oxidised DLC and phosphorus-doped DLC to support the growth and survival of primary central nervous system neurones and neuroblastoma cells. None of these substrates were cytotoxic and primary neurones adhered better to phosphorus-doped DLC than unmodified DLC. This property was used to culture cortical neurones in a predetermined micropattern. This raises the potential of DLC as a biomaterial for central nervous system (CNS) implantation. Furthermore, patterned DLC and phosphorus-doped DLC can direct neuronal growth, generating a powerful tool to study neuronal networks in a spatially distinct way. This study reports the generation of nerve cell patterns via patterned deposition of DLC.
Translated title of the contributionPatterned growth of neuronal cells on modified diamond-like carbon substrates
Original languageEnglish
Pages (from-to)2573 - 2580
Number of pages8
JournalBiomaterials
Volume29
Issue number17
DOIs
Publication statusPublished - Jun 2008

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