Peptide self-assembly measured using fluorescence correlation spectroscopy

Judith J. Mittag, Joachim O. Rädler, Jennifer J. McManus*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

2 Citations (Scopus)

Abstract

Fluorescence correlation spectroscopy (FCS) is a flexible and powerful technique to measure the diffusion of fluorescently labeled particles. It has been important in examining a range of biological processes, from intracellular transport, to DNA hybridization. It is particularly suited to measuring the assembly of peptides, since peptides are often too small to be detected by standard light scattering methods, or may not contain aromatic amino acid residues, which limits the use of other spectroscopic techniques. In this protocol, we describe state-of-the-art sample preparation for Aβ 1–42 peptide solutions and the measurement and analysis of the self-assembly of the peptide to form fibrils via a number of intermediate states using FCS.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages159-171
Number of pages13
DOIs
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1777
ISSN (Print)1064-3745

Keywords

  • Complex fluids
  • Fluorescence correlation spectroscopy
  • Gaussian distribution model
  • Peptide
  • Polydispersity
  • Self-assembly
  • Size distribution

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