Percutaneous Sampling of Local Biomolecule Gradients Across Coronary Artery Atherosclerotic Plaques

Nick E.J. West; Joseph P. Corrigan; Richard H.G. Owen; Stephen P. Hoole; Adam J. Brown; Stephen Blatcher; Andrew C. Newby

doi:10.1016/j.jacbts.2017.07.007

Percutaneous Sampling of Local Biomolecule Gradients Across Coronary Artery Atherosclerotic Plaques

Nick E.J. West, Joseph P. Corrigan, Richard H.G. Owen, Stephen P. Hoole, Adam J. Brown, Stephen Blatcher, Andrew C. Newby^*

^*Corresponding author for this work

Bristol Medical School (THS)

Research output: Contribution to journal › Article (Academic Journal) › peer-review

7 Citations (Scopus)

Abstract

A percutaneous catheter device, the Liquid Biopsy System, was developed to sample the unstirred boundary layer of blood upstream and downstream of intact and disrupted human coronary atherosclerotic plaques. Using multiplexed proximity extension assays, release of 20 biomolecules was simultaneously detected in samples taken across plaques before balloon angioplasty, including the soluble form of the endothelial lectin-like oxidized LDL receptor. Additional biomolecules, including matrix metalloproteinase-12, were released after plaque disruption with angioplasty. These experiments demonstrate the power of the Liquid Biopsy System to yield new scientific insights and its ultimate potential to generate new biomarkers and surrogate endpoints for clinical trials.

Original language	English
Pages (from-to)	646-654
Number of pages	9
Journal	JACC: Basic to Translational Science
Volume	2
Issue number	6
DOIs	https://doi.org/10.1016/j.jacbts.2017.07.007
Publication status	Published - 25 Dec 2017

Keywords

biomolecule release
coronary atherosclerosis
inflammation
vulnerable plaque

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title = "Percutaneous Sampling of Local Biomolecule Gradients Across Coronary Artery Atherosclerotic Plaques",

abstract = "A percutaneous catheter device, the Liquid Biopsy System, was developed to sample the unstirred boundary layer of blood upstream and downstream of intact and disrupted human coronary atherosclerotic plaques. Using multiplexed proximity extension assays, release of 20 biomolecules was simultaneously detected in samples taken across plaques before balloon angioplasty, including the soluble form of the endothelial lectin-like oxidized LDL receptor. Additional biomolecules, including matrix metalloproteinase-12, were released after plaque disruption with angioplasty. These experiments demonstrate the power of the Liquid Biopsy System to yield new scientific insights and its ultimate potential to generate new biomarkers and surrogate endpoints for clinical trials.",

keywords = "biomolecule release, coronary atherosclerosis, inflammation, vulnerable plaque",

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AU - West, Nick E.J.

AU - Corrigan, Joseph P.

AU - Owen, Richard H.G.

AU - Hoole, Stephen P.

AU - Brown, Adam J.

AU - Blatcher, Stephen

AU - Newby, Andrew C.

PY - 2017/12/25

Y1 - 2017/12/25

N2 - A percutaneous catheter device, the Liquid Biopsy System, was developed to sample the unstirred boundary layer of blood upstream and downstream of intact and disrupted human coronary atherosclerotic plaques. Using multiplexed proximity extension assays, release of 20 biomolecules was simultaneously detected in samples taken across plaques before balloon angioplasty, including the soluble form of the endothelial lectin-like oxidized LDL receptor. Additional biomolecules, including matrix metalloproteinase-12, were released after plaque disruption with angioplasty. These experiments demonstrate the power of the Liquid Biopsy System to yield new scientific insights and its ultimate potential to generate new biomarkers and surrogate endpoints for clinical trials.

AB - A percutaneous catheter device, the Liquid Biopsy System, was developed to sample the unstirred boundary layer of blood upstream and downstream of intact and disrupted human coronary atherosclerotic plaques. Using multiplexed proximity extension assays, release of 20 biomolecules was simultaneously detected in samples taken across plaques before balloon angioplasty, including the soluble form of the endothelial lectin-like oxidized LDL receptor. Additional biomolecules, including matrix metalloproteinase-12, were released after plaque disruption with angioplasty. These experiments demonstrate the power of the Liquid Biopsy System to yield new scientific insights and its ultimate potential to generate new biomarkers and surrogate endpoints for clinical trials.

KW - biomolecule release

KW - coronary atherosclerosis

KW - inflammation

KW - vulnerable plaque

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Percutaneous Sampling of Local Biomolecule Gradients Across Coronary Artery Atherosclerotic Plaques

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Keywords

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