Percutaneous Sampling of Local Biomolecule Gradients Across Coronary Artery Atherosclerotic Plaques

Nick E.J. West, Joseph P. Corrigan, Richard H.G. Owen, Stephen P. Hoole, Adam J. Brown, Stephen Blatcher, Andrew C. Newby*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

3 Citations (Scopus)

Abstract

A percutaneous catheter device, the Liquid Biopsy System, was developed to sample the unstirred boundary layer of blood upstream and downstream of intact and disrupted human coronary atherosclerotic plaques. Using multiplexed proximity extension assays, release of 20 biomolecules was simultaneously detected in samples taken across plaques before balloon angioplasty, including the soluble form of the endothelial lectin-like oxidized LDL receptor. Additional biomolecules, including matrix metalloproteinase-12, were released after plaque disruption with angioplasty. These experiments demonstrate the power of the Liquid Biopsy System to yield new scientific insights and its ultimate potential to generate new biomarkers and surrogate endpoints for clinical trials.

Original languageEnglish
Pages (from-to)646-654
Number of pages9
JournalJACC: Basic to Translational Science
Volume2
Issue number6
DOIs
Publication statusPublished - 25 Dec 2017

Keywords

  • biomolecule release
  • coronary atherosclerosis
  • inflammation
  • vulnerable plaque

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    West, N. E. J., Corrigan, J. P., Owen, R. H. G., Hoole, S. P., Brown, A. J., Blatcher, S., & Newby, A. C. (2017). Percutaneous Sampling of Local Biomolecule Gradients Across Coronary Artery Atherosclerotic Plaques. JACC: Basic to Translational Science, 2(6), 646-654. https://doi.org/10.1016/j.jacbts.2017.07.007