Pericyte Seeded Dual Peptide Scaffold with Improved Endothelialization for Vascular Graft Tissue Engineering

Paola Campagnolo, Adam J. Gormley, Lesley W. Chow, Anne Géraldine Guex, Paresh A. Parmar, Jennifer L. Puetzer, Joseph A.M. Steele, Alexandre Breant, Paolo Madeddu, Molly M. Stevens*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

20 Citations (Scopus)
209 Downloads (Pure)

Abstract

The development of synthetic vascular grafts for coronary artery bypass is challenged by insufficient endothelialization, which increases the risk of thrombosis, and the lack of native cellular constituents, which favors pathological remodeling. Here, a bifunctional electrospun poly(ε-caprolactone) (PCL) scaffold with potential for synthetic vascular graft applications is presented. This scaffold incorporates two tethered peptides: the osteopontin-derived peptide (Adh) on the “luminal” side and a heparin-binding peptide (Hep) on the “abluminal” side. Additionally, the “abluminal” side of the scaffold is seeded with saphenous vein-derived pericytes (SVPs) as a source of proangiogenic growth factors. The Adh peptide significantly increases endothelial cell adhesion, while the Hep peptide promotes accumulation of vascular endothelial growth factor secreted by SVPs. SVPs increase endothelial migration both in a transwell assay and a modified scratch assay performed on the PCL scaffold. Seeding of SVPs on the “abluminal”/Hep side of the scaffold further increases endothelial cell density, indicating a combinatory effect of the peptides and pericytes. Finally, SVP-seeded scaffolds are preserved by freezing in a xeno-free medium, maintaining good cell viability and function. In conclusion, this engineered scaffold combines patient-derived pericytes and spatially organized functionalities, which synergistically increase endothelial cell density and growth factor retention.

Original languageEnglish
Pages (from-to)3046-3055
Number of pages10
JournalAdvanced Healthcare Materials
Volume5
Issue number23
DOIs
Publication statusPublished - 7 Dec 2016

Keywords

  • biofunctionalization
  • electrospinning
  • endothelialization
  • pericytes
  • tissue engineered vascular graft

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