Pharmacological characterization and region-specific expression in brain of the beta 2- and beta 3-subunits of the rat GABAA receptor

S J Lolait, A M O'Carroll, K Kusano, L C Mahan

Research output: Contribution to journalArticle (Academic Journal)peer-review

50 Citations (Scopus)

Abstract

The cDNA for a third beta-subunit of the rat GABAA receptor has been cloned using another beta-subunit, which we had previously cloned [(1989) FEBS Lett. 246, 145-148], as a probe. The approximately 8-kb cDNA for this beta-subunit (termed beta 2) encodes a protein of 474 amino acid residues that shares approximately 80% sequence identity with the rat and bovine beta 1- and beta 3-subunits. Coexpression of the cloned beta-subunit cDNA with the alpha 1-subunit cDNA of the rat GABAA receptor in Xenopus oocytes produced a functional receptor and Cl- channel with pharmacological characteristics of a GABAA receptor. In contrast to interchanging alpha-subunits [(1988) Nature 335, 76-79], exchange of beta 2- or beta 3-subunits in an alpha 1/beta receptor complex did not markedly alter the pharmacological properties of expressed receptors. In situ hybridization histochemistry with synthetic subunit-specific oligo-deoxynucleotide probes revealed a region-specific expression of alpha 1-, beta 2- and beta 3-subunit mRNAs in the rat central nervous system. These observations provide an additional molecular basis for the functional heterogeneity in the GABAA receptor complex.

Original languageEnglish
Pages (from-to)17-21
Number of pages5
JournalFEBS Letters
Volume258
Issue number1
Publication statusPublished - 20 Nov 1989

Keywords

  • Animals
  • Cattle
  • Cerebral Cortex
  • Cloning, Molecular
  • DNA
  • Gene Expression
  • Histocytochemistry
  • Molecular Probe Techniques
  • Nucleic Acid Hybridization
  • Oligodeoxyribonucleotides
  • Oocytes
  • RNA, Messenger
  • Rats
  • Receptors, GABA-A
  • Xenopus
  • gamma-Aminobutyric Acid

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