Phorbol ester-evoked Ca2+ signaling in human platelets is via autocrine activation of P(2X1) receptors, not a novel non-capacitative Ca2+ entry

M T Harper; Michael J Mason; Stewart O Sage; Alan G S Harper

doi:10.1111/j.1538-7836.2010.03867.x

Phorbol ester-evoked Ca2+ signaling in human platelets is via autocrine activation of P(2X1) receptors, not a novel non-capacitative Ca2+ entry

M T Harper, Michael J Mason, Stewart O Sage, Alan G S Harper

School of Physiology, Pharmacology & Neuroscience

Research output: Contribution to journal › Article (Academic Journal) › peer-review

15 Citations (Scopus)

Abstract

Platelets are reported to possess a protein kinase C (PKC)-dependent non-capacitative Ca(2+)entry (NCCE) pathway. The phorbol ester, phorbol, 12-myristate, 13-acetate (PMA) has been suggested to stimulate platelet NCCE. Recently we demonstrated important roles in store-operated Ca(2+)entry in human platelets for Na(+)/Ca(2+) exchangers (NCXs) and autocrine signaling between platelets after dense granule secretion. As PMA evokes dense granule secretion, we have investigated the role of NCXs and autocrine signaling in PMA-evoked Ca(2+)entry.

Original language	English
Pages (from-to)	1604-13
Number of pages	10
Journal	Journal of Thrombosis and Haemostasis
Volume	8
Issue number	7
DOIs	https://doi.org/10.1111/j.1538-7836.2010.03867.x
Publication status	Published - Jul 2010

Keywords

Secretory Vesicles
Blood Platelets
Calcium
Humans
Receptors, Purinergic P2X1
Autocrine Communication
Adenosine Triphosphate
Phorbol Esters
Sodium-Calcium Exchanger
Calcium Signaling

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title = "Phorbol ester-evoked Ca2+ signaling in human platelets is via autocrine activation of P(2X1) receptors, not a novel non-capacitative Ca2+ entry",

abstract = "Platelets are reported to possess a protein kinase C (PKC)-dependent non-capacitative Ca(2+)entry (NCCE) pathway. The phorbol ester, phorbol, 12-myristate, 13-acetate (PMA) has been suggested to stimulate platelet NCCE. Recently we demonstrated important roles in store-operated Ca(2+)entry in human platelets for Na(+)/Ca(2+) exchangers (NCXs) and autocrine signaling between platelets after dense granule secretion. As PMA evokes dense granule secretion, we have investigated the role of NCXs and autocrine signaling in PMA-evoked Ca(2+)entry.",

keywords = "Secretory Vesicles, Blood Platelets, Calcium, Humans, Receptors, Purinergic P2X1, Autocrine Communication, Adenosine Triphosphate, Phorbol Esters, Sodium-Calcium Exchanger, Calcium Signaling",

author = "Harper, {M T} and Mason, {Michael J} and Sage, {Stewart O} and Harper, {Alan G S}",

year = "2010",

month = jul,

doi = "10.1111/j.1538-7836.2010.03867.x",

language = "English",

volume = "8",

pages = "1604--13",

journal = "Journal of Thrombosis and Haemostasis",

issn = "1538-7933",

publisher = "Wiley-Blackwell",

number = "7",

}

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T1 - Phorbol ester-evoked Ca2+ signaling in human platelets is via autocrine activation of P(2X1) receptors, not a novel non-capacitative Ca2+ entry

AU - Harper, M T

AU - Mason, Michael J

AU - Sage, Stewart O

AU - Harper, Alan G S

PY - 2010/7

Y1 - 2010/7

N2 - Platelets are reported to possess a protein kinase C (PKC)-dependent non-capacitative Ca(2+)entry (NCCE) pathway. The phorbol ester, phorbol, 12-myristate, 13-acetate (PMA) has been suggested to stimulate platelet NCCE. Recently we demonstrated important roles in store-operated Ca(2+)entry in human platelets for Na(+)/Ca(2+) exchangers (NCXs) and autocrine signaling between platelets after dense granule secretion. As PMA evokes dense granule secretion, we have investigated the role of NCXs and autocrine signaling in PMA-evoked Ca(2+)entry.

AB - Platelets are reported to possess a protein kinase C (PKC)-dependent non-capacitative Ca(2+)entry (NCCE) pathway. The phorbol ester, phorbol, 12-myristate, 13-acetate (PMA) has been suggested to stimulate platelet NCCE. Recently we demonstrated important roles in store-operated Ca(2+)entry in human platelets for Na(+)/Ca(2+) exchangers (NCXs) and autocrine signaling between platelets after dense granule secretion. As PMA evokes dense granule secretion, we have investigated the role of NCXs and autocrine signaling in PMA-evoked Ca(2+)entry.

KW - Secretory Vesicles

KW - Blood Platelets

KW - Calcium

KW - Humans

KW - Receptors, Purinergic P2X1

KW - Autocrine Communication

KW - Adenosine Triphosphate

KW - Phorbol Esters

KW - Sodium-Calcium Exchanger

KW - Calcium Signaling

U2 - 10.1111/j.1538-7836.2010.03867.x

DO - 10.1111/j.1538-7836.2010.03867.x

M3 - Article (Academic Journal)

C2 - 20345709

SN - 1538-7933

VL - 8

SP - 1604

EP - 1613

JO - Journal of Thrombosis and Haemostasis

JF - Journal of Thrombosis and Haemostasis

IS - 7

ER -

Phorbol ester-evoked Ca2+ signaling in human platelets is via autocrine activation of P(2X1) receptors, not a novel non-capacitative Ca2+ entry

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Keywords

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