PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE SPECIFIC PHOSPHOLIPASE-C IN PHARBITIS-NIL MEMBRANES

P L R BONNER, S L PRIOR, A M HETHERINGTON, P J LUMSDEN

Research output: Contribution to journalArticle (Academic Journal)peer-review

3 Citations (Scopus)

Abstract

Phosphatidylinositol 4,5-bisphosphate specific phospholipase C has been detected in a membrane preparation from Pharbitis nil cotyledons. The enzyme has a pH optimum of 6.8 and activated by calcium ions, deoxycholate, phosphatidylinositol and phosphatidylethanolamine. The enzyme is inhibited to varying degrees by Tween 20, Triton X100, zinc, copper, cobalt and manganese ions and phosphatidylserine. G-protein activators do not affect the activity of Pharbitis nil phospholipase C. Analysis of the products of the reaction by HPLC shows inositol 1,4,5-trisphosphate from phospholipase C and inositol bisphosphate from inositol-1 and -5 phosphatase activity.

Original languageEnglish
Pages (from-to)367-372
Number of pages6
JournalBiologia plantarum
Volume34
Issue number5-6
Publication statusPublished - 1992

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