Phosphorylation of TFIIB links transcription initiation and termination

Y Wang, JA Fairley, SGE Roberts

Research output: Contribution to journalArticle (Academic Journal)peer-review

41 Citations (Scopus)

Abstract

The general transcription factor TFIIB plays a central role in preinitiation complex (PIC) assembly and the recruitment of RNA polymerase II (RNA pol II) to the promoter. Recent studies have revealed that TFIIB engages in contact with the transcription termination region and also with complexes that are involved in 3' end processing and/or termination. Here we report that TFIIB can be phosphorylated within the N terminus at serine 65 in vivo and that the phosphorylated form of TFIIB is present within (PICs). Surprisingly, TFIIB serine 65 phosphorylation is required after the phosphorylation of serine 5 of RNA pol II C-terminal domain (CTD) has occurred, but before productive transcription initiation begins. We show that phosphorylation of TFIIB at serine 65 regulates the interaction between TFIIB and the CstF-64 component of the CstF 3' cleavage and polyadenylation complex. This directs the recruitment of CstF (cleavage stimulatory factor) to the terminator and also the recruitment of the CstF and CPSF (cleavage and polyadenylation specific factor) complexes to the promoter. Our results reveal that phosphorylation of TFIIB is a critical event in transcription that links the gene promoter and terminator and triggers initiation by RNA pol II.
Translated title of the contributionPhosphorylation of TFIIB links transcription initiation and termination
Original languageEnglish
Pages (from-to)548 - 553
Number of pages6
JournalCurrent Biology
Volume20
Issue number6
DOIs
Publication statusPublished - 11 Mar 2010

Fingerprint Dive into the research topics of 'Phosphorylation of TFIIB links transcription initiation and termination'. Together they form a unique fingerprint.

Cite this