PICK1 links Argonaute 2 to endosomes in neuronal dendrites and regulates miRNA activity.

Anna Antoniou; Marcio Baptista; Nick Carney; Jonathan Hanley

doi:10.1002/embr.201337631

PICK1 links Argonaute 2 to endosomes in neuronal dendrites and regulates miRNA activity.

Anna Antoniou, Marcio Baptista, Nick Carney, Jonathan Hanley

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Abstract

MicroRNAs fine-tune gene expression by inhibiting the translation of mRNA targets. Argonaute (Ago) proteins are critical mediators of microRNA-induced post-transcriptional silencing and have been shown to associate with endosomal compartments, but the molecular mechanisms that underlie this process are unclear, especially in neurons. Here, we report a novel interaction between Ago2 and the BAR-domain protein, PICK1. We show that PICK1 promotes Ago2 localization at endosomal compartments in neuronal dendrites and inhibits Ago2 function in translational repression following neuronal stimulation. We propose that PICK1 provides a link between activity-dependent endosomal trafficking and local regulation of translation in neurons.

Original language	English
Pages (from-to)	548-556
Number of pages	9
Journal	EMBO Reports
Volume	15
Issue number	5
Early online date	10 Apr 2014
DOIs	https://doi.org/10.1002/embr.201337631
Publication status	Published - 1 May 2014

Keywords

RISC
synaptic plasticity
trafficking

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Regulation of Arp2/3-mediated actin polymerisation by PICK1 in neuronal function.
Hanley, J. G. (Principal Investigator)
10/01/11 → 10/06/14
Project: Research

Professor Jonathan G Hanley
- Jon.Hanleybristol.acuk
- School of Biochemistry - Professor of Molecular Neuroscience
- Dynamic Cell Biology
- Bristol Neuroscience
Person: Academic , Member

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title = "PICK1 links Argonaute 2 to endosomes in neuronal dendrites and regulates miRNA activity.",

abstract = "MicroRNAs fine-tune gene expression by inhibiting the translation of mRNA targets. Argonaute (Ago) proteins are critical mediators of microRNA-induced post-transcriptional silencing and have been shown to associate with endosomal compartments, but the molecular mechanisms that underlie this process are unclear, especially in neurons. Here, we report a novel interaction between Ago2 and the BAR-domain protein, PICK1. We show that PICK1 promotes Ago2 localization at endosomal compartments in neuronal dendrites and inhibits Ago2 function in translational repression following neuronal stimulation. We propose that PICK1 provides a link between activity-dependent endosomal trafficking and local regulation of translation in neurons.",

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AU - Antoniou, Anna

AU - Baptista, Marcio

AU - Carney, Nick

AU - Hanley, Jonathan

PY - 2014/5/1

Y1 - 2014/5/1

N2 - MicroRNAs fine-tune gene expression by inhibiting the translation of mRNA targets. Argonaute (Ago) proteins are critical mediators of microRNA-induced post-transcriptional silencing and have been shown to associate with endosomal compartments, but the molecular mechanisms that underlie this process are unclear, especially in neurons. Here, we report a novel interaction between Ago2 and the BAR-domain protein, PICK1. We show that PICK1 promotes Ago2 localization at endosomal compartments in neuronal dendrites and inhibits Ago2 function in translational repression following neuronal stimulation. We propose that PICK1 provides a link between activity-dependent endosomal trafficking and local regulation of translation in neurons.

AB - MicroRNAs fine-tune gene expression by inhibiting the translation of mRNA targets. Argonaute (Ago) proteins are critical mediators of microRNA-induced post-transcriptional silencing and have been shown to associate with endosomal compartments, but the molecular mechanisms that underlie this process are unclear, especially in neurons. Here, we report a novel interaction between Ago2 and the BAR-domain protein, PICK1. We show that PICK1 promotes Ago2 localization at endosomal compartments in neuronal dendrites and inhibits Ago2 function in translational repression following neuronal stimulation. We propose that PICK1 provides a link between activity-dependent endosomal trafficking and local regulation of translation in neurons.

KW - RISC

KW - synaptic plasticity

KW - trafficking

U2 - 10.1002/embr.201337631

DO - 10.1002/embr.201337631

M3 - Article (Academic Journal)

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SN - 1469-221X

VL - 15

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EP - 556

JO - EMBO Reports

JF - EMBO Reports

IS - 5

ER -

PICK1 links Argonaute 2 to endosomes in neuronal dendrites and regulates miRNA activity.

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Keywords

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Regulation of Arp2/3-mediated actin polymerisation by PICK1 in neuronal function.

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Professor Jonathan G Hanley

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