Polymerization in the actin ATPase clan regulates hexokinase activity in yeast

Patrick R. Stoddard, Eric M. Lynch, Daniel P. Farrell, Annie M. Dosey, Frank DiMaio, Tom A. Williams, Justin M. Kollman, Andrew W. Murray*, Ethan Garner*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

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Abstract

The actin fold is found in cytoskeletal polymers, chaperones, and various metabolic enzymes. Many actin-fold proteins, such as the carbohydrate kinases, do not polymerize. We found that Glk1, a Saccharomyces cerevisiae glucokinase, forms two-stranded filaments with ultrastructure that is distinct from that of cytoskeletal polymers. In cells, Glk1 polymerized upon sugar addition and depolymerized upon sugar withdrawal. Polymerization inhibits enzymatic activity; the Glk1 monomer-polymer equilibrium sets a maximum rate of glucose phosphorylation regardless of Glk1 concentration. A mutation that eliminated Glk1 polymerization alleviated concentration-dependent enzyme inhibition. Yeast containing nonpolymerizing Glk1 were less fit when growing on sugars and more likely to die when refed glucose. Glk1 polymerization arose independently from other actin-related filaments and may allow yeast to rapidly modulate glucokinase activity as nutrient availability changes.
Original languageEnglish
Pages (from-to)1039-1042
Number of pages4
JournalScience
Volume367
Issue number6481
DOIs
Publication statusPublished - 28 Feb 2020

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