Polyphenol-Rich Cranberry Extracts Modulate Virulence of Streptococcus mutans-Candida albicans Biofilms Implicated in the Pathogenesis of Early Childhood Caries

Nebu Philip, Shaneen J Leishman, H M H N Bandara, Laurence J Walsh

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Purpose: The purpose of this study was to investigate the effects of polyphenol-rich cranberry extracts on dual-species Streptococcus mutans-Candida. albicans biofilms implicated in contributing to the severity of early childhood caries. 

Methods:S. mutans-C. albicans biofilms were grown on saliva-coated hydroxyapatite discs (s-HA) mounted on the high-throughput Amsterdam Active Attachment model. The s-HA discs were treated with the cranberry extracts/vehicle control for five minutes just before biofilm growth and subsequently, for similar exposure times, after 12 hours and 24 hours of biofilm growth. The treated 24-hour-old biofilms were then assessed for acidogenicity, metabolic activity, exopolysaccharide (EPS)/microbial biovolumes, structural organization, and colony forming unit (CFU) counts. Results: Treatment with 500 to 1,000 μg/mL of the cranberry extracts produced significant reductions in acidogenicity and metabolic activity (P<0.0001) compared to the control-treated biofilms. A significant decrease in biovolumes of the EPS (P=0.003) and microbial biofilm components (P=0.007) was also seen. Qualitative assessment of confocal biofilm images revealed that the cranberry extract disrupted biofilm structural architecture. Finally, significantly fewer S. mutans (P=0.006) and C. albicans (P=0.036) CFUs were recovered from the cranberry-treated biofilms than from the control-treated bio-films.

Conclusions: Cranberry extracts inhibited cariogenic virulence properties of S. mutans-C. albicans dual-species biofilms in an in vitro model.

Original languageEnglish
Pages (from-to)56-62
Number of pages7
JournalPediatric Dentistry
Volume41
Issue number1
Publication statusPublished - 15 Jan 2019

Keywords

  • Biofilms/drug effects
  • Candida albicans/drug effects
  • Child
  • Dental Caries/prevention & control
  • Fruit/chemistry
  • Humans
  • Microbial Sensitivity Tests
  • Plant Extracts/pharmacology
  • Polyphenols/pharmacology
  • Streptococcus mutans/drug effects
  • Vaccinium macrocarpon/chemistry
  • Virulence/drug effects

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