Polyurethane scaffolds seeded with CD34+ cells maintain early stem cells whilst also facilitating prolonged egress of haematopoietic progenitors

Charlie E Severn, Hugo Macedo, Mark Eagle, Paul Rooney, Athanasios Mantalaris, Ash M Toye

Research output: Contribution to journalArticle (Academic Journal)peer-review

14 Citations (Scopus)
311 Downloads (Pure)


We describe a 3D erythroid culture system that utilises a porous polyurethane (PU) scaffold to mimic the compartmentalisation found in the bone marrow. PU scaffolds seeded with peripheral blood CD34+ cells exhibit a remarkable reproducibility of egress, with an increased output when directly compared to human bone scaffolds over 28 days. Immunofluorescence demonstrated the persistence of CD34+ cells within the scaffolds for the entirety of the culture. To characterise scaffold outputs, we designed a flow cytometry panel that utilises surface marker expression observed in standard 2D erythroid and megakaryocyte cultures. This showed that the egress population is comprised of haematopoietic progenitor cells (CD36+)GPA-/low). Control cultures conducted in parallel but in the absence of a scaffold were also generally maintained for the longevity of the culture albeit with a higher level of cell death. The harvested scaffold egress can also be expanded and differentiated to the reticulocyte stage. In summary, PU scaffolds can behave as a subtractive compartmentalised culture system retaining and allowing maintenance of the seeded "CD34+ cell" population despite this population decreasing in amount as the culture progresses, whilst also facilitating egress of increasingly differentiated cells.
Original languageEnglish
Article number32149
Number of pages12
JournalScientific Reports
Publication statusPublished - 30 Aug 2016


  • Haematopoietic stem cells
  • Stem-cell differentiation

Fingerprint Dive into the research topics of 'Polyurethane scaffolds seeded with CD34<sup>+</sup> cells maintain early stem cells whilst also facilitating prolonged egress of haematopoietic progenitors'. Together they form a unique fingerprint.

Cite this