(Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes

Maria V Neguembor, Ruben Sebastian-Perez, Francesco Aulicino, Pablo A Gomez-Garcia, Maria P Cosma, Melike Lakadamyali

Research output: Contribution to journalArticle (Academic Journal)peer-review

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Abstract

CRISPR/dCas9-based labeling has allowed direct visualization of genomic regions in living cells. However, poor labeling efficiency and signal-to-background ratio have limited its application to visualize genome organization using super-resolution microscopy. We developed (Po)STAC (Polycistronic SunTAg modified CRISPR) by combining CRISPR/dCas9 with SunTag labeling and polycistronic vectors. (Po)STAC enhances both labeling efficiency and fluorescence signal detected from labeled loci enabling live cell imaging as well as super-resolution fixed-cell imaging of multiple genes with high spatiotemporal resolution.

Original languageEnglish
Article numbere30
Number of pages10
JournalNucleic Acids Research
Volume46
Issue number5
DOIs
Publication statusPublished - 16 Mar 2018

Keywords

  • cell biology
  • targeted gene modification

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