Postprandial response of adiponectin, interleukin-6, tumor necrosis factor-alpha, and C-reactive protein to a high-fat dietary load

Sally D Poppitt, Geraldine F Keogh, Fiona E Lithander, Yu Wang, Tom B Mulvey, Yih-Kai Chan, Brian H McArdle, Garth J S Cooper

Research output: Contribution to journalArticle (Academic Journal)peer-review

82 Citations (Scopus)


OBJECTIVE: Circulating levels of adiponectin are low in obesity and metabolic disorders associated with increasing fat mass including insulin resistance and dyslipidemia. Body fat stores may be positively related to intake of dietary fat, but little is known of mechanisms by which serum adiponectin may be regulated through diet. We investigated acute effects of a high-fat load and changes in fatty acid saturation on circulating adiponectin and associated mediators of inflammation including interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and C-reactive protein (CRP).

METHODS: A high-fat test meal (59 +/- 4 g fat; 71% of energy as fat) containing a high ( approximately 71:29) or low ( approximately 55:45) ratio of saturated:unsaturated fatty acids was given at breakfast on two occasions. Blood samples were collected at 0 (baseline), 1, 3, and 6 h for measurement of adiponectin, IL-6, TNF-alpha, and high-sensitivity CRP. A fat-exclusion lunch, snack, and dinner were also given and blood samples collected at 10 and 24 h.

RESULTS: Eighteen healthy, lean men completed the trial. There was no evidence of acute change in circulating adiponectin in response to the lipid bolus or a differential effect of fatty acid saturation on adiponectin, high-sensitivity CRP, or IL-6 (P > 0.05). IL-6 increased over 6 h on both treatments (time, P < 0.05). TNF-alpha decreased on the high saturated:unsaturated fatty acid treatment (treatment by time, P < 0.05). There were no significant correlations between circulating adiponectin and insulin on either dietary treatment in these normoglycemic subjects.

CONCLUSION: Acute changes in the content of saturated and unsaturated fatty acids had no adverse effect on postprandial circulation of the adipose-related factors adiponectin, IL-6, TNF-alpha, or high-sensitivity CRP.

Original languageEnglish
Pages (from-to)322-9
Number of pages8
Issue number4
Publication statusPublished - Apr 2008


  • Adiponectin
  • Adult
  • Area Under Curve
  • Biomarkers
  • C-Reactive Protein
  • Cross-Over Studies
  • Dietary Fats
  • Dietary Fats, Unsaturated
  • Double-Blind Method
  • Humans
  • Interleukin-6
  • Male
  • Postprandial Period
  • Time Factors
  • Treatment Outcome
  • Tumor Necrosis Factor-alpha
  • Journal Article
  • Randomized Controlled Trial

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