OBJECTIVE: To evaluate the antimicrobial efficacy of berberine, a plant alkaloid.
METHODS: Five multi-drug resistant (MDR) STEC/EPEC and five MDR ETEC isolates from yaks with haemorrhagic diarrhoea were selected for the study. Antibacterial activity of berberine was evaluated by broth dilution and disc diffusion methods. The binding kinetics of berberine to DNA and protein was also enumerated.
RESULTS: For both categories of enterovirulent Escherichia coli (E. coli) isolates, berberine displayed the antibacterial effect in a dose dependent manner. The MIC(50) of berberine chloride for STEC/EPEC isolates varied from 2.07 μM to 3.6 μM with a mean of (2.95 ± 0.33) μM where as for ETEC strains it varied from 1.75 to 1.96 μM with a mean of (1.87 ± 0.03) μM. Berberine bind more tightly with double helix DNA with Bmax and Kd of (24.68±2.62) and (357.8±57.8), respectively. Berberine reacted with protein in comparatively loose manner with Bmax and Kd of (18.9±3.83) and (286.2±113.6), respectively.
CONCLUSIONS: The results indicate clearly that berberine may serve as a good antibacterial against multi drug resistant E. coli.
Bibliographical noteCopyright © 2013 Hainan Medical College. Published by Elsevier B.V. All rights reserved.
- Anti-Bacterial Agents/therapeutic use
- Cattle Diseases/drug therapy
- DNA, Bacterial/metabolism
- Diarrhea/drug therapy
- Drug Resistance, Multiple, Bacterial
- Enteropathogenic Escherichia coli
- Enterotoxigenic Escherichia coli
- Escherichia coli Infections/drug therapy
- Microbial Sensitivity Tests
- Protein Binding
- Shiga-Toxigenic Escherichia coli