Potential role of Alternaria and Cladosporium species in canine lymphoplasmacytic rhinitis

E. Mercier; I. R. Peters; F. Billen; G. Battaille; C. Clercx; M. J. Day; D. Peeters

doi:10.1111/jsap.12049

Potential role of Alternaria and Cladosporium species in canine lymphoplasmacytic rhinitis

E. Mercier^*, I. R. Peters, F. Billen, G. Battaille, C. Clercx, M. J. Day, D. Peeters

^*Corresponding author for this work

Langford Third Party

Research output: Contribution to journal › Article (Academic Journal) › peer-review

10 Citations (Scopus)

Abstract

Objectives To evaluate the possible role of Alternaria and Cladosporium species in the pathogenesis of canine lymphoplasmacytic rhinitis by comparing the amount of specific fungal DNA in nasal mucosal biopsies between dogs without nasal neoplasia and those with lymphoplasmacytic rhinitis or nasal neoplasia. Methods Quantitative real-time polymerase chain reaction (qPCR) assays detecting DNA from Alternaria and Cladosporium fungi were applied to nasal mucosal biopsies collected from dogs with lymphoplasmacytic rhinitis (n=8), dogs with nasal neoplasia (n=10) and control animals (n=10). A copy number for each sample was calculated using a standard curve of known copy number and differences amongst groups were assessed using KruskalWallis tests. Results No significant difference was found between the groups. Low levels of Alternaria DNA (10100 copies/PCR) were detected in one sample; very low levels of DNA (

Original language	English
Pages (from-to)	179-183
Number of pages	5
Journal	Journal of Small Animal Practice
Volume	54
Issue number	4
DOIs	https://doi.org/10.1111/jsap.12049
Publication status	Published - Apr 2013

Keywords

FUNGAL DNA
NASAL POLYPS
TISSUE
ANTIFUNGAL TREATMENT
POLYMERASE-CHAIN-REACTION
SINONASAL ASPERGILLOSIS
MESSENGER-RNA EXPRESSION
SINUSITIS
CHRONIC RHINOSINUSITIS
AMPHOTERICIN-B IRRIGATION

Access to Document

10.1111/jsap.12049

Cite this

@article{1f3cf5caad564896805042f1fb75af85,

title = "Potential role of Alternaria and Cladosporium species in canine lymphoplasmacytic rhinitis",

abstract = "Objectives To evaluate the possible role of Alternaria and Cladosporium species in the pathogenesis of canine lymphoplasmacytic rhinitis by comparing the amount of specific fungal DNA in nasal mucosal biopsies between dogs without nasal neoplasia and those with lymphoplasmacytic rhinitis or nasal neoplasia. Methods Quantitative real-time polymerase chain reaction (qPCR) assays detecting DNA from Alternaria and Cladosporium fungi were applied to nasal mucosal biopsies collected from dogs with lymphoplasmacytic rhinitis (n=8), dogs with nasal neoplasia (n=10) and control animals (n=10). A copy number for each sample was calculated using a standard curve of known copy number and differences amongst groups were assessed using KruskalWallis tests. Results No significant difference was found between the groups. Low levels of Alternaria DNA (10100 copies/PCR) were detected in one sample; very low levels of DNA (",

keywords = "FUNGAL DNA, NASAL POLYPS, TISSUE, ANTIFUNGAL TREATMENT, POLYMERASE-CHAIN-REACTION, SINONASAL ASPERGILLOSIS, MESSENGER-RNA EXPRESSION, SINUSITIS, CHRONIC RHINOSINUSITIS, AMPHOTERICIN-B IRRIGATION",

author = "E. Mercier and Peters, {I. R.} and F. Billen and G. Battaille and C. Clercx and Day, {M. J.} and D. Peeters",

year = "2013",

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doi = "10.1111/jsap.12049",

language = "English",

volume = "54",

pages = "179--183",

journal = "Journal of Small Animal Practice",

issn = "0022-4510",

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TY - JOUR

T1 - Potential role of Alternaria and Cladosporium species in canine lymphoplasmacytic rhinitis

AU - Mercier, E.

AU - Peters, I. R.

AU - Billen, F.

AU - Battaille, G.

AU - Clercx, C.

AU - Day, M. J.

AU - Peeters, D.

PY - 2013/4

Y1 - 2013/4

N2 - Objectives To evaluate the possible role of Alternaria and Cladosporium species in the pathogenesis of canine lymphoplasmacytic rhinitis by comparing the amount of specific fungal DNA in nasal mucosal biopsies between dogs without nasal neoplasia and those with lymphoplasmacytic rhinitis or nasal neoplasia. Methods Quantitative real-time polymerase chain reaction (qPCR) assays detecting DNA from Alternaria and Cladosporium fungi were applied to nasal mucosal biopsies collected from dogs with lymphoplasmacytic rhinitis (n=8), dogs with nasal neoplasia (n=10) and control animals (n=10). A copy number for each sample was calculated using a standard curve of known copy number and differences amongst groups were assessed using KruskalWallis tests. Results No significant difference was found between the groups. Low levels of Alternaria DNA (10100 copies/PCR) were detected in one sample; very low levels of DNA (

AB - Objectives To evaluate the possible role of Alternaria and Cladosporium species in the pathogenesis of canine lymphoplasmacytic rhinitis by comparing the amount of specific fungal DNA in nasal mucosal biopsies between dogs without nasal neoplasia and those with lymphoplasmacytic rhinitis or nasal neoplasia. Methods Quantitative real-time polymerase chain reaction (qPCR) assays detecting DNA from Alternaria and Cladosporium fungi were applied to nasal mucosal biopsies collected from dogs with lymphoplasmacytic rhinitis (n=8), dogs with nasal neoplasia (n=10) and control animals (n=10). A copy number for each sample was calculated using a standard curve of known copy number and differences amongst groups were assessed using KruskalWallis tests. Results No significant difference was found between the groups. Low levels of Alternaria DNA (10100 copies/PCR) were detected in one sample; very low levels of DNA (

KW - FUNGAL DNA

KW - NASAL POLYPS

KW - TISSUE

KW - ANTIFUNGAL TREATMENT

KW - POLYMERASE-CHAIN-REACTION

KW - SINONASAL ASPERGILLOSIS

KW - MESSENGER-RNA EXPRESSION

KW - SINUSITIS

KW - CHRONIC RHINOSINUSITIS

KW - AMPHOTERICIN-B IRRIGATION

U2 - 10.1111/jsap.12049

DO - 10.1111/jsap.12049

M3 - Article (Academic Journal)

C2 - 23496099

VL - 54

SP - 179

EP - 183

JO - Journal of Small Animal Practice

JF - Journal of Small Animal Practice

SN - 0022-4510

IS - 4

ER -