Abstract
Objectives To evaluate the possible role of Alternaria and Cladosporium species in the pathogenesis of canine lymphoplasmacytic rhinitis by comparing the amount of specific fungal DNA in nasal mucosal biopsies between dogs without nasal neoplasia and those with lymphoplasmacytic rhinitis or nasal neoplasia. Methods Quantitative real-time polymerase chain reaction (qPCR) assays detecting DNA from Alternaria and Cladosporium fungi were applied to nasal mucosal biopsies collected from dogs with lymphoplasmacytic rhinitis (n=8), dogs with nasal neoplasia (n=10) and control animals (n=10). A copy number for each sample was calculated using a standard curve of known copy number and differences amongst groups were assessed using KruskalWallis tests. Results No significant difference was found between the groups. Low levels of Alternaria DNA (10100 copies/PCR) were detected in one sample; very low levels of DNA (
| Original language | English |
|---|---|
| Pages (from-to) | 179-183 |
| Number of pages | 5 |
| Journal | Journal of Small Animal Practice |
| Volume | 54 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 2013 |
Keywords
- FUNGAL DNA
- NASAL POLYPS
- TISSUE
- ANTIFUNGAL TREATMENT
- POLYMERASE-CHAIN-REACTION
- SINONASAL ASPERGILLOSIS
- MESSENGER-RNA EXPRESSION
- SINUSITIS
- CHRONIC RHINOSINUSITIS
- AMPHOTERICIN-B IRRIGATION