Preliminary demonstration of benchtop NMR metabolic profiling of feline urine:  chronic kidney disease as a case study

Natalie C Finch; Benita Percival; Elena Hunter; Robin J. Blagg; Emily-Jayne Blackwell; Severine Tasker; Philippe Wilson; et al.

doi:10.1186/s13104-021-05888-y

Preliminary demonstration of benchtop NMR metabolic profiling of feline urine: chronic kidney disease as a case study

Natalie C Finch, Benita Percival, Elena Hunter, Robin J. Blagg, Emily-Jayne Blackwell, Severine Tasker, Philippe Wilson^*, et al.

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

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Abstract

Objective:
The use of benchtop metabolic profiling technology based on nuclear magnetic resonance (NMR) was evaluated in a small cohort of cats with a view to applying this as a viable and rapid metabolic tool to support clinical decision making.

Results:
Urinary metabolites were analysed from four subjects consisting of two healthy controls and two chronic kidney disease (CKD) IRIS stage 2 cases. The study identified 15 metabolites in cats with CKD that were different from the controls. Among them were acetate, creatinine, citrate, taurine, glycine, serine and threonine. Benchtop NMR technology is capable of distinguishing between chronic kidney disease case and control samples in a pilot feline cohort based on metabolic profile. We offer perspectives on the further development of this pilot work and the potential of the technology, when combined with sample databases and computational intelligence techniques to offer a clinical decision support tool not only for cases of renal disease but other metabolic conditions in the future.

Original language	English
Article number	469
Number of pages	5
Journal	BMC Research Notes
Volume	14
DOIs	https://doi.org/10.1186/s13104-021-05888-y
Publication status	Published - 24 Dec 2021

Bibliographical note

Publisher Copyright:
© The Author(s) 2021.

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title = "Preliminary demonstration of benchtop NMR metabolic profiling of feline urine: chronic kidney disease as a case study",

abstract = "Objective:The use of benchtop metabolic profiling technology based on nuclear magnetic resonance (NMR) was evaluated in a small cohort of cats with a view to applying this as a viable and rapid metabolic tool to support clinical decision making.Results:Urinary metabolites were analysed from four subjects consisting of two healthy controls and two chronic kidney disease (CKD) IRIS stage 2 cases. The study identified 15 metabolites in cats with CKD that were different from the controls. Among them were acetate, creatinine, citrate, taurine, glycine, serine and threonine. Benchtop NMR technology is capable of distinguishing between chronic kidney disease case and control samples in a pilot feline cohort based on metabolic profile. We offer perspectives on the further development of this pilot work and the potential of the technology, when combined with sample databases and computational intelligence techniques to offer a clinical decision support tool not only for cases of renal disease but other metabolic conditions in the future.",

author = "Finch, \{Natalie C\} and Benita Percival and Elena Hunter and Blagg, \{Robin J.\} and Emily-Jayne Blackwell and Severine Tasker and Philippe Wilson and et al.",

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doi = "10.1186/s13104-021-05888-y",

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T2 - chronic kidney disease as a case study

AU - Finch, Natalie C

AU - Percival, Benita

AU - Hunter, Elena

AU - Blagg, Robin J.

AU - Blackwell, Emily-Jayne

AU - Tasker, Severine

AU - Wilson, Philippe

AU - al., et

PY - 2021/12/24

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N2 - Objective:The use of benchtop metabolic profiling technology based on nuclear magnetic resonance (NMR) was evaluated in a small cohort of cats with a view to applying this as a viable and rapid metabolic tool to support clinical decision making.Results:Urinary metabolites were analysed from four subjects consisting of two healthy controls and two chronic kidney disease (CKD) IRIS stage 2 cases. The study identified 15 metabolites in cats with CKD that were different from the controls. Among them were acetate, creatinine, citrate, taurine, glycine, serine and threonine. Benchtop NMR technology is capable of distinguishing between chronic kidney disease case and control samples in a pilot feline cohort based on metabolic profile. We offer perspectives on the further development of this pilot work and the potential of the technology, when combined with sample databases and computational intelligence techniques to offer a clinical decision support tool not only for cases of renal disease but other metabolic conditions in the future.

AB - Objective:The use of benchtop metabolic profiling technology based on nuclear magnetic resonance (NMR) was evaluated in a small cohort of cats with a view to applying this as a viable and rapid metabolic tool to support clinical decision making.Results:Urinary metabolites were analysed from four subjects consisting of two healthy controls and two chronic kidney disease (CKD) IRIS stage 2 cases. The study identified 15 metabolites in cats with CKD that were different from the controls. Among them were acetate, creatinine, citrate, taurine, glycine, serine and threonine. Benchtop NMR technology is capable of distinguishing between chronic kidney disease case and control samples in a pilot feline cohort based on metabolic profile. We offer perspectives on the further development of this pilot work and the potential of the technology, when combined with sample databases and computational intelligence techniques to offer a clinical decision support tool not only for cases of renal disease but other metabolic conditions in the future.

U2 - 10.1186/s13104-021-05888-y

DO - 10.1186/s13104-021-05888-y

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