Preliminary kinetic analysis of acyl carrier protein-ketoacylsynthase interactions in the actinorhodin minimal polyketide synthase

Pedro Beltran-Alvarez, Christopher J. Arthur, Russell J. Cox, John Crosby, Matthew P. Crump, Thomas J. Simpson

Research output: Contribution to journalArticle (Academic Journal)

5 Citations (Scopus)

Abstract

Interactions between the acyl carrier protein (ACP) and ketoacylsynthase (KS) components of the actinorhodin polyketide synthase have been investigated using kinetic assays. These indicate that for three different quantifiable interactions (acceleration of self-malonylation, initiation and extension) mutations of E47 and E53 residues located on ACP helix II have different effects. Initiation clearly involves interaction between KS beta and ACP helix II, but self-malonylation acceleration and extension by KS alpha appear not to be affected strongly by the same mutations.

Original languageEnglish
Pages (from-to)511-518
Number of pages8
JournalMolecular bioSystems
Volume5
Issue number5
DOIs
Publication statusPublished - 2009

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