PREPARATION OF MONOCLONAL-ANTIBODIES SPECIFIC FOR THE SUBCLASSES OF CANINE IGG

G MAZZA, A H WHITING, M J DAY, W P H DUFFUS

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Canine IgG is composed of four subclasses, which are defined as IgG(1), IgG(2), IgG(3) and IgG(4) on the basis of data from fast protein liquid chromatography, and their electrophoretic mobilities and relative concentrations in serum. This paper describes the preparation of mAbs specific for determinants on canine IgG(2), IgG(3) and IgG(4). The mAb specific for IgG(2) resulted from a conventional immunisation protocol. The mAb specific for IgG(3) was a result of immunisation with IgG(3) combined with the suppression of the immune response to IgG(1) by passively administered anti-IgG(1) antibody. The mAb specific for IgG(4) resulted from immunisation with Fab or Fc fragments which were obtained by the cleavage of the IgG(4) molecule with papain. The specificity of each mAb was established by using an enzyme-linked immunosorbent assay which showed that all three specific clones recognised a determinant in the Fd region of the canine immunoglobulin molecule.

Original languageEnglish
Pages (from-to)140-145
Number of pages6
JournalResearch in Veterinary Science
Volume57
Issue number2
Publication statusPublished - Sep 1994

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