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Preservation of feather fibers from the Late Cretaceous dinosaur Shuvuuia deserti raises concern about immunohistochemical analyses on fossils

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Preservation of feather fibers from the Late Cretaceous dinosaur Shuvuuia deserti raises concern about immunohistochemical analyses on fossils. / Saitta, Evan T.; Fletcher, Ian; Martin, Peter George; Pittman, Michael; Kaye, Thomas G.; True, Lawrence D.; Norell, Mark A.; Abbott, Geoffrey D.; Summons, Roger E.; Penkman, Kirsty; Vinther, Jakob.

In: Organic Geochemistry, Vol. 125, 11.2018, p. 142-151.

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Saitta, ET, Fletcher, I, Martin, PG, Pittman, M, Kaye, TG, True, LD, Norell, MA, Abbott, GD, Summons, RE, Penkman, K & Vinther, J 2018, 'Preservation of feather fibers from the Late Cretaceous dinosaur Shuvuuia deserti raises concern about immunohistochemical analyses on fossils', Organic Geochemistry, vol. 125, pp. 142-151. https://doi.org/10.1016/j.orggeochem.2018.09.008

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Saitta, Evan T. ; Fletcher, Ian ; Martin, Peter George ; Pittman, Michael ; Kaye, Thomas G. ; True, Lawrence D. ; Norell, Mark A. ; Abbott, Geoffrey D. ; Summons, Roger E. ; Penkman, Kirsty ; Vinther, Jakob. / Preservation of feather fibers from the Late Cretaceous dinosaur Shuvuuia deserti raises concern about immunohistochemical analyses on fossils. In: Organic Geochemistry. 2018 ; Vol. 125. pp. 142-151.

Bibtex

@article{70a5baa665654925a2113b446ec6e227,
title = "Preservation of feather fibers from the Late Cretaceous dinosaur Shuvuuia deserti raises concern about immunohistochemical analyses on fossils",
abstract = "White fibers from a Late Cretaceous dinosaur Shuvuuia deserti stained positive for β-keratin antibodies in a 1999 paper, followed by many similar immunological claims for Mesozoic protein in bones and integument. Antibodies recognize protein epitopes derived from its tertiary and quaternary structure, so such results would suggest long polypeptide preservation allowing for sequencing with palaeobiological implications. However, proteins are relatively unstable biomacromolecules that readily hydrolyze and amino acids exhibit predictable instability under diagenetic heat and pressure. Furthermore, antibodies can yield false positives. We reanalyzed a Shuvuuia fiber using focused ion beam scanning electron microscopy, energy-dispersive X-ray spectroscopy, time-of-flight secondary ion mass spectrometry, and laser-stimulated fluorescence imaging, finding it to be inorganic and composed mainly of calcium phosphate. Our findings are inconsistent with any protein or other original organic substance preservation in the Shuvuuia fiber, suggesting that immunohistochemistry may be inappropriate for analyzing fossils due to issues with false positives and a lack of controls.",
keywords = "Antibodies, Calcium phosphate, Feathers, Fossils, Keratin, Protein",
author = "Saitta, {Evan T.} and Ian Fletcher and Martin, {Peter George} and Michael Pittman and Kaye, {Thomas G.} and True, {Lawrence D.} and Norell, {Mark A.} and Abbott, {Geoffrey D.} and Summons, {Roger E.} and Kirsty Penkman and Jakob Vinther",
year = "2018",
month = "11",
doi = "10.1016/j.orggeochem.2018.09.008",
language = "English",
volume = "125",
pages = "142--151",
journal = "Organic Geochemistry",
issn = "0146-6380",
publisher = "Elsevier",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - Preservation of feather fibers from the Late Cretaceous dinosaur Shuvuuia deserti raises concern about immunohistochemical analyses on fossils

AU - Saitta, Evan T.

AU - Fletcher, Ian

AU - Martin, Peter George

AU - Pittman, Michael

AU - Kaye, Thomas G.

AU - True, Lawrence D.

AU - Norell, Mark A.

AU - Abbott, Geoffrey D.

AU - Summons, Roger E.

AU - Penkman, Kirsty

AU - Vinther, Jakob

PY - 2018/11

Y1 - 2018/11

N2 - White fibers from a Late Cretaceous dinosaur Shuvuuia deserti stained positive for β-keratin antibodies in a 1999 paper, followed by many similar immunological claims for Mesozoic protein in bones and integument. Antibodies recognize protein epitopes derived from its tertiary and quaternary structure, so such results would suggest long polypeptide preservation allowing for sequencing with palaeobiological implications. However, proteins are relatively unstable biomacromolecules that readily hydrolyze and amino acids exhibit predictable instability under diagenetic heat and pressure. Furthermore, antibodies can yield false positives. We reanalyzed a Shuvuuia fiber using focused ion beam scanning electron microscopy, energy-dispersive X-ray spectroscopy, time-of-flight secondary ion mass spectrometry, and laser-stimulated fluorescence imaging, finding it to be inorganic and composed mainly of calcium phosphate. Our findings are inconsistent with any protein or other original organic substance preservation in the Shuvuuia fiber, suggesting that immunohistochemistry may be inappropriate for analyzing fossils due to issues with false positives and a lack of controls.

AB - White fibers from a Late Cretaceous dinosaur Shuvuuia deserti stained positive for β-keratin antibodies in a 1999 paper, followed by many similar immunological claims for Mesozoic protein in bones and integument. Antibodies recognize protein epitopes derived from its tertiary and quaternary structure, so such results would suggest long polypeptide preservation allowing for sequencing with palaeobiological implications. However, proteins are relatively unstable biomacromolecules that readily hydrolyze and amino acids exhibit predictable instability under diagenetic heat and pressure. Furthermore, antibodies can yield false positives. We reanalyzed a Shuvuuia fiber using focused ion beam scanning electron microscopy, energy-dispersive X-ray spectroscopy, time-of-flight secondary ion mass spectrometry, and laser-stimulated fluorescence imaging, finding it to be inorganic and composed mainly of calcium phosphate. Our findings are inconsistent with any protein or other original organic substance preservation in the Shuvuuia fiber, suggesting that immunohistochemistry may be inappropriate for analyzing fossils due to issues with false positives and a lack of controls.

KW - Antibodies

KW - Calcium phosphate

KW - Feathers

KW - Fossils

KW - Keratin

KW - Protein

UR - http://www.scopus.com/inward/record.url?scp=85053807015&partnerID=8YFLogxK

U2 - 10.1016/j.orggeochem.2018.09.008

DO - 10.1016/j.orggeochem.2018.09.008

M3 - Article

VL - 125

SP - 142

EP - 151

JO - Organic Geochemistry

JF - Organic Geochemistry

SN - 0146-6380

ER -