Prevalence and genotypes of Giardia duodenalis in dairy and beef cattle in farms around Charlottetown, Prince Edward Island, Canada

Fabienne D Uehlinger; Spencer J Greenwood; Ryan O'Handley; J Trenton McClure; Tatjana Coklin; Brent R Dixon; Melvin de Boer; Hester Zwiers; Herman W Barkema

Prevalence and genotypes of Giardia duodenalis in dairy and beef cattle in farms around Charlottetown, Prince Edward Island, Canada

Fabienne D Uehlinger, Spencer J Greenwood, Ryan O'Handley, J Trenton McClure, Tatjana Coklin, Brent R Dixon, Melvin de Boer, Hester Zwiers, Herman W Barkema

Research output: Contribution to journal › Article (Academic Journal) › peer-review

17 Citations (Scopus)

Abstract

Prevalence of Giardia duodenalis in dairy and beef cattle on farms around Charlottetown, Prince Edward Island (Canada) was determined by analyzing feces using direct immunofluorescence antibody microscopy. Genotypes were determined by 16S-rRNA sequencing. Fecal samples (n = 892) were collected from adult cattle in dairy tie-stall, dairy free-stall, and beef herds (10 herds each), and from calves (n = 183) from 11 dairy farms. Prevalence rates were 38% and 51% in cows and calves, respectively. Giardia duodenalis was present in all dairy herds, in 9/10 beef herds and in calves from 10/11 herds examined. Prevalence rates were 40% and 41% for cows in tie- and free-stall herds, respectively, and 27% for beef cows. Zoonotic Assemblage A was found in 12.2% of calves concomitantly infected with Assemblage E. All successfully sequenced samples (114/128) from cows corresponded to Assemblage E. Giardia duodenalis is highly prevalent in cattle herds in Prince Edward Island and Assemblage A in calves is a potential public health concern.

Original language	English
Pages (from-to)	967-72
Number of pages	6
Journal	Canadian Veterinary Journal
Volume	52
Issue number	9
Publication status	Published - Sept 2011

Keywords

RNA, Ribosomal, 16S
Animals
Giardia lamblia
Giardiasis
Prince Edward Island
Genotype
Cattle Diseases
Zoonoses
Cattle
Public Health
Feces
Female
Male
Prevalence

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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24th World Buiatrics Congress, Nice (France)
Fabienne D Uehlinger (Speaker)
15 Oct 2006 → 19 Oct 2006
Activity: Participating in or organising an event types › Participation in conference
4th International Giardia Conference & 1st combined Giardia/Cryptosporidium meeting, Amsterdam (The Netherlands)
Fabienne D Uehlinger (Speaker)
20 Sept 2004 → 24 Sept 2004
Activity: Participating in or organising an event types › Participation in conference
23rd World Buiatric Congress, Quebec City (Canada)
Fabienne D Uehlinger (Speaker)
11 Jul 2004 → 16 Jul 2004
Activity: Participating in or organising an event types › Participation in conference

Cite this

@article{5e005bb744d04e34aabf8713bc98a8e8,

title = "Prevalence and genotypes of Giardia duodenalis in dairy and beef cattle in farms around Charlottetown, Prince Edward Island, Canada",

abstract = "Prevalence of Giardia duodenalis in dairy and beef cattle on farms around Charlottetown, Prince Edward Island (Canada) was determined by analyzing feces using direct immunofluorescence antibody microscopy. Genotypes were determined by 16S-rRNA sequencing. Fecal samples (n = 892) were collected from adult cattle in dairy tie-stall, dairy free-stall, and beef herds (10 herds each), and from calves (n = 183) from 11 dairy farms. Prevalence rates were 38% and 51% in cows and calves, respectively. Giardia duodenalis was present in all dairy herds, in 9/10 beef herds and in calves from 10/11 herds examined. Prevalence rates were 40% and 41% for cows in tie- and free-stall herds, respectively, and 27% for beef cows. Zoonotic Assemblage A was found in 12.2% of calves concomitantly infected with Assemblage E. All successfully sequenced samples (114/128) from cows corresponded to Assemblage E. Giardia duodenalis is highly prevalent in cattle herds in Prince Edward Island and Assemblage A in calves is a potential public health concern.",

keywords = "RNA, Ribosomal, 16S, Animals, Giardia lamblia, Giardiasis, Prince Edward Island, Genotype, Cattle Diseases, Zoonoses, Cattle, Public Health, Feces, Female, Male, Prevalence",

author = "Uehlinger, {Fabienne D} and Greenwood, {Spencer J} and Ryan O'Handley and McClure, {J Trenton} and Tatjana Coklin and Dixon, {Brent R} and {de Boer}, Melvin and Hester Zwiers and Barkema, {Herman W}",

year = "2011",

month = sep,

language = "English",

volume = "52",

pages = "967--72",

journal = "Canadian Veterinary Journal",

issn = "0008-5286",

publisher = "Canadian Veterinary Medical Association",

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}

TY - JOUR

T1 - Prevalence and genotypes of Giardia duodenalis in dairy and beef cattle in farms around Charlottetown, Prince Edward Island, Canada

AU - Uehlinger, Fabienne D

AU - Greenwood, Spencer J

AU - O'Handley, Ryan

AU - McClure, J Trenton

AU - Coklin, Tatjana

AU - Dixon, Brent R

AU - de Boer, Melvin

AU - Zwiers, Hester

AU - Barkema, Herman W

PY - 2011/9

Y1 - 2011/9

N2 - Prevalence of Giardia duodenalis in dairy and beef cattle on farms around Charlottetown, Prince Edward Island (Canada) was determined by analyzing feces using direct immunofluorescence antibody microscopy. Genotypes were determined by 16S-rRNA sequencing. Fecal samples (n = 892) were collected from adult cattle in dairy tie-stall, dairy free-stall, and beef herds (10 herds each), and from calves (n = 183) from 11 dairy farms. Prevalence rates were 38% and 51% in cows and calves, respectively. Giardia duodenalis was present in all dairy herds, in 9/10 beef herds and in calves from 10/11 herds examined. Prevalence rates were 40% and 41% for cows in tie- and free-stall herds, respectively, and 27% for beef cows. Zoonotic Assemblage A was found in 12.2% of calves concomitantly infected with Assemblage E. All successfully sequenced samples (114/128) from cows corresponded to Assemblage E. Giardia duodenalis is highly prevalent in cattle herds in Prince Edward Island and Assemblage A in calves is a potential public health concern.

AB - Prevalence of Giardia duodenalis in dairy and beef cattle on farms around Charlottetown, Prince Edward Island (Canada) was determined by analyzing feces using direct immunofluorescence antibody microscopy. Genotypes were determined by 16S-rRNA sequencing. Fecal samples (n = 892) were collected from adult cattle in dairy tie-stall, dairy free-stall, and beef herds (10 herds each), and from calves (n = 183) from 11 dairy farms. Prevalence rates were 38% and 51% in cows and calves, respectively. Giardia duodenalis was present in all dairy herds, in 9/10 beef herds and in calves from 10/11 herds examined. Prevalence rates were 40% and 41% for cows in tie- and free-stall herds, respectively, and 27% for beef cows. Zoonotic Assemblage A was found in 12.2% of calves concomitantly infected with Assemblage E. All successfully sequenced samples (114/128) from cows corresponded to Assemblage E. Giardia duodenalis is highly prevalent in cattle herds in Prince Edward Island and Assemblage A in calves is a potential public health concern.

KW - RNA, Ribosomal, 16S

KW - Animals

KW - Giardia lamblia

KW - Giardiasis

KW - Prince Edward Island

KW - Genotype

KW - Cattle Diseases

KW - Zoonoses

KW - Cattle

KW - Public Health

KW - Feces

KW - Female

KW - Male

KW - Prevalence

M3 - Article (Academic Journal)

C2 - 22379195

SN - 0008-5286

VL - 52

SP - 967

EP - 972

JO - Canadian Veterinary Journal

JF - Canadian Veterinary Journal

IS - 9

ER -

Prevalence and genotypes of Giardia duodenalis in dairy and beef cattle in farms around Charlottetown, Prince Edward Island, Canada

Abstract

Keywords

UN SDGs

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Activities

24th World Buiatrics Congress, Nice (France)

4th International Giardia Conference & 1st combined Giardia/Cryptosporidium meeting, Amsterdam (The Netherlands)

23rd World Buiatric Congress, Quebec City (Canada)

Cite this