Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation

Jonas R Knudsen, Lorna R Hodgson, Paul Verkade, et al.

Research output: Contribution to journalArticle (Academic Journal)peer-review

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Abstract

Objective
Exercise is a cornerstone in the management of skeletal muscle insulin-resistance. A well-established benefit of a single bout of exercise is increased insulin sensitivity for hours post-exercise in the previously exercised musculature. Although rodent studies suggest that the insulin-sensitization phenomenon involves enhanced insulin-stimulated GLUT4 cell surface translocation and might involve intramuscular redistribution of GLUT4, the conservation to humans is unknown.

Methods
Healthy young males underwent an insulin-sensitizing one-legged kicking exercise bout for 1 h followed by fatigue bouts to exhaustion. Muscle biopsies were obtained 4 h post-exercise before and after a 2-hour hyperinsulinemic-euglycemic clamp.

Results
A detailed microscopy-based analysis of GLUT4 distribution muscle specimen in seven different myocellular compartments revealed that prior exercise increased GLUT4 localization in insulin-responsive storage vesicles and T-tubuli. Furthermore, insulin-stimulated GLUT4 localization was augmented at the sarcolemma and in the endosomal compartments.

Conclusions
An intracellular redistribution of GLUT4 post-exercise is proposed as a molecular mechanism contributing to the insulin-sensitizing effect of prior exercise in human skeletal muscle.
Original languageEnglish
Article number100998
Number of pages9
JournalMolecular metabolism
Volume39
Early online date17 Apr 2020
DOIs
Publication statusPublished - 1 Sep 2020

Keywords

  • Exercise
  • skeletal muscle
  • GLUT4
  • insulin sensitivity
  • insulin-resistance

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