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Probing the Dynamic Nature of Self-Assembling Cyclic Peptide–Polymer Nanotubes in Solution and in Mammalian Cells

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Probing the Dynamic Nature of Self-Assembling Cyclic Peptide–Polymer Nanotubes in Solution and in Mammalian Cells. / Rho, Julia Y.; Brendel, Johannes C.; MacFarlane, Liam R.; Mansfield, Edward D.H.; Peltier, Raoul; Rogers, Sarah; Hartlieb, Matthias; Perrier, Sébastien.

In: Advanced Functional Materials, Vol. 28, No. 24, 1704569, 13.06.2018.

Research output: Contribution to journalArticle

Harvard

Rho, JY, Brendel, JC, MacFarlane, LR, Mansfield, EDH, Peltier, R, Rogers, S, Hartlieb, M & Perrier, S 2018, 'Probing the Dynamic Nature of Self-Assembling Cyclic Peptide–Polymer Nanotubes in Solution and in Mammalian Cells', Advanced Functional Materials, vol. 28, no. 24, 1704569. https://doi.org/10.1002/adfm.201704569

APA

Rho, J. Y., Brendel, J. C., MacFarlane, L. R., Mansfield, E. D. H., Peltier, R., Rogers, S., ... Perrier, S. (2018). Probing the Dynamic Nature of Self-Assembling Cyclic Peptide–Polymer Nanotubes in Solution and in Mammalian Cells. Advanced Functional Materials, 28(24), [1704569]. https://doi.org/10.1002/adfm.201704569

Vancouver

Rho JY, Brendel JC, MacFarlane LR, Mansfield EDH, Peltier R, Rogers S et al. Probing the Dynamic Nature of Self-Assembling Cyclic Peptide–Polymer Nanotubes in Solution and in Mammalian Cells. Advanced Functional Materials. 2018 Jun 13;28(24). 1704569. https://doi.org/10.1002/adfm.201704569

Author

Rho, Julia Y. ; Brendel, Johannes C. ; MacFarlane, Liam R. ; Mansfield, Edward D.H. ; Peltier, Raoul ; Rogers, Sarah ; Hartlieb, Matthias ; Perrier, Sébastien. / Probing the Dynamic Nature of Self-Assembling Cyclic Peptide–Polymer Nanotubes in Solution and in Mammalian Cells. In: Advanced Functional Materials. 2018 ; Vol. 28, No. 24.

Bibtex

@article{f0b95ca93a014c458dcd616d55291f86,
title = "Probing the Dynamic Nature of Self-Assembling Cyclic Peptide–Polymer Nanotubes in Solution and in Mammalian Cells",
abstract = "Self-assembling cyclic peptide–polymer nanotubes have emerged as a fascinating supramolecular system, well suited for a diverse range of biomedical applications. Due to their well-defined diameter, tunable peptide anatomy, and ability to disassemble in situ, they have been investigated as promising materials for numerous applications including biosensors, antimicrobials, and drug delivery. Despite this continuous effort, the underlying mechanisms of assembly and disassembly are still not fully understood. In particular, the exchange of units between individual assembled nanotubes has been overlooked so far, despite its knowledge being essential for understanding their behavior in different environments. To investigate the dynamic nature of these systems, cyclic peptide–polymer nanotubes are synthesized, conjugated with complementary dyes, which undergo a F{\"o}rster resonance energy transfer (FRET) in close proximity. Model conjugates enable to demonstrate not only that their self-assembly is highly dynamic and not kinetically trapped, but also that the self-assembly of the conjugates is strongly influenced by both solvent and concentration. Additionally, the versatility of the FRET system allows studying the dynamic exchange of these systems in mammalian cells in vitro using confocal microscopy, demonstrating the exchange of subunits between assembled nanotubes in the highly complex environment of a cell.",
keywords = "cyclic peptide nanotubes, F{\"o}rster resonance energy transfer, peptide–polymer conjugates, self-assembly, supramolecular polymers",
author = "Rho, {Julia Y.} and Brendel, {Johannes C.} and MacFarlane, {Liam R.} and Mansfield, {Edward D.H.} and Raoul Peltier and Sarah Rogers and Matthias Hartlieb and S{\'e}bastien Perrier",
year = "2018",
month = "6",
day = "13",
doi = "10.1002/adfm.201704569",
language = "English",
volume = "28",
journal = "Advanced Functional Materials",
issn = "1616-301X",
publisher = "Wiley-VCH Verlag",
number = "24",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - Probing the Dynamic Nature of Self-Assembling Cyclic Peptide–Polymer Nanotubes in Solution and in Mammalian Cells

AU - Rho, Julia Y.

AU - Brendel, Johannes C.

AU - MacFarlane, Liam R.

AU - Mansfield, Edward D.H.

AU - Peltier, Raoul

AU - Rogers, Sarah

AU - Hartlieb, Matthias

AU - Perrier, Sébastien

PY - 2018/6/13

Y1 - 2018/6/13

N2 - Self-assembling cyclic peptide–polymer nanotubes have emerged as a fascinating supramolecular system, well suited for a diverse range of biomedical applications. Due to their well-defined diameter, tunable peptide anatomy, and ability to disassemble in situ, they have been investigated as promising materials for numerous applications including biosensors, antimicrobials, and drug delivery. Despite this continuous effort, the underlying mechanisms of assembly and disassembly are still not fully understood. In particular, the exchange of units between individual assembled nanotubes has been overlooked so far, despite its knowledge being essential for understanding their behavior in different environments. To investigate the dynamic nature of these systems, cyclic peptide–polymer nanotubes are synthesized, conjugated with complementary dyes, which undergo a Förster resonance energy transfer (FRET) in close proximity. Model conjugates enable to demonstrate not only that their self-assembly is highly dynamic and not kinetically trapped, but also that the self-assembly of the conjugates is strongly influenced by both solvent and concentration. Additionally, the versatility of the FRET system allows studying the dynamic exchange of these systems in mammalian cells in vitro using confocal microscopy, demonstrating the exchange of subunits between assembled nanotubes in the highly complex environment of a cell.

AB - Self-assembling cyclic peptide–polymer nanotubes have emerged as a fascinating supramolecular system, well suited for a diverse range of biomedical applications. Due to their well-defined diameter, tunable peptide anatomy, and ability to disassemble in situ, they have been investigated as promising materials for numerous applications including biosensors, antimicrobials, and drug delivery. Despite this continuous effort, the underlying mechanisms of assembly and disassembly are still not fully understood. In particular, the exchange of units between individual assembled nanotubes has been overlooked so far, despite its knowledge being essential for understanding their behavior in different environments. To investigate the dynamic nature of these systems, cyclic peptide–polymer nanotubes are synthesized, conjugated with complementary dyes, which undergo a Förster resonance energy transfer (FRET) in close proximity. Model conjugates enable to demonstrate not only that their self-assembly is highly dynamic and not kinetically trapped, but also that the self-assembly of the conjugates is strongly influenced by both solvent and concentration. Additionally, the versatility of the FRET system allows studying the dynamic exchange of these systems in mammalian cells in vitro using confocal microscopy, demonstrating the exchange of subunits between assembled nanotubes in the highly complex environment of a cell.

KW - cyclic peptide nanotubes

KW - Förster resonance energy transfer

KW - peptide–polymer conjugates

KW - self-assembly

KW - supramolecular polymers

UR - http://www.scopus.com/inward/record.url?scp=85033238434&partnerID=8YFLogxK

U2 - 10.1002/adfm.201704569

DO - 10.1002/adfm.201704569

M3 - Article

AN - SCOPUS:85033238434

VL - 28

JO - Advanced Functional Materials

JF - Advanced Functional Materials

SN - 1616-301X

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ER -