Probiotic lactobacilli inhibit early stages of Candida albicans biofilm development by reducing their growth, cell adhesion, and filamentation

Victor Haruo Matsubara, Yi Wang, H M H N Bandara, Marcia Pinto Alves Mayer, Lakshman P Samaranayake

Research output: Contribution to journalArticle (Academic Journal)peer-review

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Abstract

We evaluated the inhibitory effects of the probiotic Lactobacillus species on different phases of Candida albicans biofilm development. Quantification of biofilm growth and ultrastructural analyses were performed on C. albicans biofilms treated with Lactobacillus rhamnosus, Lactobacillus casei, and Lactobacillus acidophilus planktonic cell suspensions as well as their supernatants. Planktonic lactobacilli induced a significant reduction (p < 0.05) in the number of biofilm cells (25.5-61.8 %) depending on the probiotic strain and the biofilm phase. L. rhamnosus supernatants had no significant effect on the mature biofilm (p > 0.05), but significantly reduced the early stages of Candida biofilm formation (p < 0.01). Microscopic analyses revealed that L. rhamnosus suspensions reduced Candida hyphal differentiation, leading to a predominance of budding growth. All lactobacilli negatively impacted C. albicans yeast-to-hyphae differentiation and biofilm formation. The inhibitory effects of the probiotic Lactobacillus on C. albicans entailed both cell-cell interactions and secretion of exometabolites that may impact on pathogenic attributes associated with C. albicans colonization on host surfaces and yeast filamentation. This study clarifies, for the first time, the mechanics of how Lactobacillus species may antagonize C. albicans host colonization. Our data elucidate the inhibitory mechanisms that define the probiotic candicidal activity of lactobacilli, thus supporting their utility as an adjunctive therapeutic mode against mucosal candidal infections.

Original languageEnglish
Pages (from-to)6415-6426
Number of pages12
JournalApplied Microbiology and Biotechnology
Volume100
Issue number14
DOIs
Publication statusPublished - 18 Apr 2016

Keywords

  • Antibiosis
  • Biofilms
  • Candida albicans
  • Cell Adhesion
  • Culture Media
  • Hyphae
  • Lactobacillus acidophilus
  • Lactobacillus rhamnosus
  • Probiotics
  • Journal Article

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