Production of transgenic rodents by the microinjection of cloned DNA into fertilized one-cell eggs

S L Si-Hoe, S Wells, D Murphy

    Research output: Contribution to journalArticle (Academic Journal)peer-review

    11 Citations (Scopus)

    Abstract

    Transgenic technologies that enable rapid movement between genotype and phenotype through specific loss-of-function, overexpression, or misexpression phenotypes will be crucial in the elucidation of gene sequences emerging from genome projects. This article describes detailed procedures for the generation of transgenic mice and rats by the injection of cloned DNA into the pronuclei of fertilized one-cell eggs.

    Original languageEnglish
    Pages (from-to)151-82
    Number of pages32
    JournalMolecular Biotechnology
    Volume17
    Issue number2
    DOIs
    Publication statusPublished - Feb 2001

    Keywords

    • Animal Husbandry
    • Animal Welfare
    • Animals
    • Animals, Genetically Modified
    • Cryopreservation
    • DNA
    • Embryo Transfer
    • Female
    • Fertilization in Vitro
    • Gene Expression
    • Genetic Techniques
    • Male
    • Mice
    • Mice, Transgenic
    • Microinjections
    • Models, Genetic
    • Ovulation Induction
    • Pregnancy
    • Rats
    • Vasectomy
    • Zygote

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