Production of transgenic rodents by the microinjection of cloned DNA into fertilized one-cell eggs

S L Si-Hoe; S Wells; D Murphy

doi:10.1385/MB:17:2:151

Production of transgenic rodents by the microinjection of cloned DNA into fertilized one-cell eggs

S L Si-Hoe, S Wells, D Murphy

Research output: Contribution to journal › Article (Academic Journal) › peer-review

13 Citations (Scopus)

Abstract

Transgenic technologies that enable rapid movement between genotype and phenotype through specific loss-of-function, overexpression, or misexpression phenotypes will be crucial in the elucidation of gene sequences emerging from genome projects. This article describes detailed procedures for the generation of transgenic mice and rats by the injection of cloned DNA into the pronuclei of fertilized one-cell eggs.

Original language	English
Pages (from-to)	151-82
Number of pages	32
Journal	Molecular Biotechnology
Volume	17
Issue number	2
DOIs	https://doi.org/10.1385/MB:17:2:151
Publication status	Published - Feb 2001

Keywords

Animal Husbandry
Animal Welfare
Animals
Animals, Genetically Modified
Cryopreservation
DNA
Embryo Transfer
Female
Fertilization in Vitro
Gene Expression
Genetic Techniques
Male
Mice
Mice, Transgenic
Microinjections
Models, Genetic
Ovulation Induction
Pregnancy
Rats
Vasectomy
Zygote

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title = "Production of transgenic rodents by the microinjection of cloned DNA into fertilized one-cell eggs",

abstract = "Transgenic technologies that enable rapid movement between genotype and phenotype through specific loss-of-function, overexpression, or misexpression phenotypes will be crucial in the elucidation of gene sequences emerging from genome projects. This article describes detailed procedures for the generation of transgenic mice and rats by the injection of cloned DNA into the pronuclei of fertilized one-cell eggs.",

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author = "Si-Hoe, {S L} and S Wells and D Murphy",

year = "2001",

month = feb,

doi = "10.1385/MB:17:2:151",

language = "English",

volume = "17",

pages = "151--82",

journal = "Molecular Biotechnology",

issn = "1073-6085",

publisher = "Humana Press",

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TY - JOUR

T1 - Production of transgenic rodents by the microinjection of cloned DNA into fertilized one-cell eggs

AU - Si-Hoe, S L

AU - Wells, S

AU - Murphy, D

PY - 2001/2

Y1 - 2001/2

N2 - Transgenic technologies that enable rapid movement between genotype and phenotype through specific loss-of-function, overexpression, or misexpression phenotypes will be crucial in the elucidation of gene sequences emerging from genome projects. This article describes detailed procedures for the generation of transgenic mice and rats by the injection of cloned DNA into the pronuclei of fertilized one-cell eggs.

AB - Transgenic technologies that enable rapid movement between genotype and phenotype through specific loss-of-function, overexpression, or misexpression phenotypes will be crucial in the elucidation of gene sequences emerging from genome projects. This article describes detailed procedures for the generation of transgenic mice and rats by the injection of cloned DNA into the pronuclei of fertilized one-cell eggs.

KW - Animal Husbandry

KW - Animal Welfare

KW - Animals

KW - Animals, Genetically Modified

KW - Cryopreservation

KW - DNA

KW - Embryo Transfer

KW - Female

KW - Fertilization in Vitro

KW - Gene Expression

KW - Genetic Techniques

KW - Male

KW - Mice

KW - Mice, Transgenic

KW - Microinjections

KW - Models, Genetic

KW - Ovulation Induction

KW - Pregnancy

KW - Rats

KW - Vasectomy

KW - Zygote

U2 - 10.1385/MB:17:2:151

DO - 10.1385/MB:17:2:151

M3 - Article (Academic Journal)

C2 - 11395864

SN - 1073-6085

VL - 17

SP - 151

EP - 182

JO - Molecular Biotechnology

JF - Molecular Biotechnology

IS - 2

ER -

Production of transgenic rodents by the microinjection of cloned DNA into fertilized one-cell eggs

Abstract

Keywords

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