Programmed assembly of synthetic protocells into thermoresponsive prototissues

Pierangelo Gobbo, Avinash J. Patil, Mei Li, Robert Harniman, Wuge H. Briscoe, Stephen Mann*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

31 Citations (Scopus)
405 Downloads (Pure)

Abstract

Although several new types of synthetic cell-like entities are now available, their structural integration into spatially interlinked prototissues that communicate and display coordinated functions remains a considerable challenge. Here we describe the programmed assembly of synthetic prototissue constructs based on the bio-orthogonal adhesion of a spatially confined binary community of protein–polymer protocells, termed proteinosomes. The thermoresponsive properties of the interlinked proteinosomes are used collectively to generate prototissue spheroids capable of reversible contractions that can be enzymatically modulated and exploited for mechanochemical transduction. Overall, our methodology opens up a route to the fabrication of artificial tissue-like materials capable of collective behaviours, and addresses important emerging challenges in bottom-up synthetic biology and bioinspired engineering.

Original languageEnglish
Pages (from-to)1145-1153
Number of pages9
JournalNature Materials
Volume17
Issue number12
Early online date8 Oct 2018
DOIs
Publication statusPublished - 1 Dec 2018

Structured keywords

  • BrisSynBio
  • Bristol BioDesign Institute

Keywords

  • SYNTHETIC BIOLOGY

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