Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats

Maarten P Koeners, Connie P C Ow, David M Russell, Roger G Evans, Simon C Malpas

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

Abstract

A relative deficiency in kidney oxygenation, i.e., renal hypoxia, may contribute to the initiation and progression of acute and chronic kidney disease. A critical barrier to investigate this is the lack of methods allowing measurement of the partial pressure of oxygen in kidney tissue for long periods in vivo. We have developed, validated, and tested a novel telemetric method that can do this. Here we provide details on the calibration, implantation, implementation for data recording, and reuse of this telemetry-based technology for measurement of medullary tissue oxygen tension in conscious, unrestrained rats. This technique provides an important additional tool for investigating the impact of renal hypoxia in biology and pathophysiology.

Original languageEnglish
Title of host publicationKidney Research
Subtitle of host publicationExperimental Protocols
EditorsTim D Hewitson, Edward R Smith, Stephen G Holt
PublisherSpringer, New York, NY
Pages93-111
Number of pages19
ISBN (Electronic)9781493933532
ISBN (Print)9781493933518
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1397
ISSN (Print)1064-3745

Keywords

  • kidney
  • tissue oxygen concentration
  • medulla
  • telemetry

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