Abstract
Insulin-Degrading-Enzyme (IDE) is a Zn2+-dependent peptidase
highly conserved throughout evolution and ubiquitously distributed in
mammalian tissues wherein it displays a prevalent cytosolic
localization. We have recently demonstrated a novel Heat Shock
Protein-like behaviour of IDE and its association with the 26S
proteasome. In the present study, we examine the mechanistic and
molecular features of IDE-26S proteasome interaction in a cell
experimental model, extending the investigation also to the effect of
IDE on the enzymatic activities of the 26S proteasome. Further, kinetic
investigations indicate that the 26S proteasome activity undergoes a
functional modulation by IDE through an extra-catalytic mechanism. The
IDE-26S proteasome interaction was analyzed during the Heat Shock
Response and we report novel findings on IDE intracellular distribution
that might be of critical relevance for cell metabolism.
Original language | English |
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Article number | e0132455 |
Number of pages | 21 |
Journal | PLoS ONE |
Volume | 10 |
Issue number | 7 |
DOIs | |
Publication status | Published - 17 Jul 2015 |
Keywords
- Blotting, Western
- Cell Line, Tumor
- Complex Mixtures
- Endoplasmic Reticulum
- Gene Silencing
- Heat-Shock Response
- Humans
- Insulysin
- Neuroblastoma
- Proteasome Endopeptidase Complex
- Protein Binding
- Stress, Physiological
- Subcellular Fractions
- Time Factors
- Journal Article
- Research Support, Non-U.S. Gov't