Projects per year
Abstract
Astrocytic tumors are the most common form of primary brain tumor. Astrocytic tumor cells infiltrate the surrounding CNS tissue, allowing them to evade removal upon surgical resection of the primary tumor. Dynamic changes to the actin cytoskeleton are crucial to cancer cell invasion, but the specific mechanisms that underlie the particularly invasive phenotype of astrocytic tumor cells are unclear. Protein interacting with C kinase 1 (PICK1) is a PDZ and BAR domain–containing protein that inhibits actin-related protein 2/3 (Arp2/3)-dependent actin polymerization and is involved in regulating the trafficking of a number of cell-surface receptors. Here we report that, in contrast to other cancers, PICK1 expression is down-regulated in grade IV astrocytic tumor cell lines and also in clinical cases of the disease in which grade IV tumors have progressed from lower-grade tumors. Exogenous expression of PICK1 in the grade IV astrocytic cell line U251 reduces their capacity for anchorage-independent growth, two-dimensional migration, and invasion through a three-dimensional matrix, strongly suggesting that low PICK1 expression plays an important role in astrocytic tumorigenesis. We propose that PICK1 negatively regulates neoplastic infiltration of astrocytic tumors and that manipulation of PICK1 is an attractive possibility for therapeutic intervention.
Original language | English |
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Pages (from-to) | 4552-4561 |
Number of pages | 10 |
Journal | Molecular Biology of the Cell |
Volume | 26 |
Issue number | 5 |
Early online date | 14 Oct 2015 |
DOIs | |
Publication status | Published - 15 Dec 2015 |
Research Groups and Themes
- Cerebrovascular and Dementia Research Group
Fingerprint
Dive into the research topics of 'Protein interacting with C kinase 1 suppresses invasion and anchorage independent growth of astrocytic tumour cells'. Together they form a unique fingerprint.Projects
- 1 Finished
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Neuronal vulnerability to ischaemia: the role of AMPA receptor trafficking
Hanley, J. G. (Principal Investigator)
1/04/14 → 1/04/17
Project: Research
Activities
- 1 Public talk, debate, discussion
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University of Bristol's 3 minute thesis competition
Cockbill, L. M. R. (Participant)
6 Jun 2014Activity: Talk or presentation types › Public talk, debate, discussion
Equipment
Profiles
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Professor Jonathan G Hanley
- School of Biochemistry - Professor of Molecular Neuroscience
- Dynamic Cell Biology
- Bristol Neuroscience
Person: Academic , Member