Abstract

While proteins have been treated as particles with a spherically symmetric interaction, of course in reality the situation is rather more complex. A simple step towards higher complexity is to treat the proteins as non–spherical particles and
that is the approach we pursue here. We investigate the phase behavior of enhanced green fluorescent protein (eGFP) under the addition of a non–adsorbing polymer, polyethylene glycol (PEG). From small angle x-ray scattering we infer that the eGFP undergoes dimerization and we treat the dimers as spherocylinders with aspect ratio L/D − 1 = 1.05. Despite the complex nature of the proteins, we find that the phase behaviour is similar to that of hard spherocylinders with ideal polymer depletant, exhibiting aggregation and, in a small region of the phase diagram, crystallization. By comparing our measurements of the onset of aggregation with predictions for hard colloids and ideal polymers [S.V. Savenko and M. Dijkstra, J. Chem. Phys 124, 234902 (2006) and F. lo Verso et al., Phys. Rev. E 73, 061407 (2006)] we find good agreement, which suggests that the eGFP proteins are consistent with hard spherocylinders and ideal polymer.
Original languageEnglish
JournalJournal of Chemical Physics
Publication statusAccepted/In press - 10 Aug 2021

Fingerprint

Dive into the research topics of 'Protein-Polymer Mixtures in the Colloid Limit: Aggregation, Sedimentation and Crystallization'. Together they form a unique fingerprint.

Cite this