Protocol for in vitro co-culture, proliferation, and cell cycle analyses of patient-derived leukemia cells

Jessica Parker, Sean Hockney, Carly Knill, David McDonald, Andrew Filby, Deepali Pal*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Leukemia niche impacts quiescence; however, culturing patient-derived samples ex vivo is technically challenging. Here, we present a protocol for in vitro co-culture of patient-derived xenograft acute lymphoblastic leukemia (PDX-ALL) cells with human mesenchymal stem cells (MSCs). We describe steps for labeling PDX-ALL cells with CellTrace Violet dye to demonstrate MSC-primed PDX-ALL cycling. We then detail procedures to identify MSC-primed G0/quiescent PDX-ALL cells via Hoechst-33342/Pyronin Y live cell cycle analysis.

 For complete details on the use and execution of this protocol, please refer to Pal et al.1,2.

Original languageEnglish
Article number103202
Pages (from-to)1-18
Number of pages18
JournalSTAR Protocols
Volume5
Issue number3
Early online date20 Jul 2024
DOIs
Publication statusPublished - 20 Sept 2024

Bibliographical note

Publisher Copyright:
© 2024 The Authors

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